Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is a useful tool for the synthesis of native acyl-[acyl-carrier-protein] for use in examining other lipid biosynthetic enzymes in E. coli | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | 0.015 | saturated fatty acids | C12:0 to C16:0 | Escherichia coli | |
0.03 | 0.16 | unsaturated fatty acids | fatty acids with shorter chain length than 12 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | - |
Escherichia coli | - |
- |
membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Salt | requirement for high concentrations of salt, with the exception of Mg2+, the requirement for ion is nonspecific. Chaotrophic salts are effective at lower concentrations than less chaotrophic salts | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | enzyme may function to reintroduce free fatty acids into the biosynthetic pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + an acid + [acyl-carrier protein] | the best substrates are saturated fatty acids, C12:0 to C16:0 | Escherichia coli | AMP + diphosphate + acyl-[acyl-carrier protein] | - |
? | |
ATP + palmitic acid + [acyl-carrier protein] | - |
Escherichia coli | AMP + diphosphate + palmitoyl-[acyl-carrier protein] | - |
? | |
ATP + saturated fatty acids + an [acyl-carrier protein] | - |
Escherichia coli | ? | - |
? | |
ATP + unsaturated fatty acids + an [acyl-carrier protein] | - |
Escherichia coli | ? | - |
? | |
additional information | enzyme may function to reintroduce free fatty acids into the biosynthetic pathway | Escherichia coli | ? | - |
? |