Literature summary for 6.2.1.20 extracted from

Jiang, Y.; Chan, C.H.; Cronan, J.E.
The soluble acyl-acyl carrier protein synthetase of Vibrio harveyi B392 is a member of the medium chain acyl-CoA synthetase family (2006), Biochemistry, 45, 10008-10019.

Search for more literature for 6.2.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21(DELTADE3)	Vibrio harveyi

Protein Variants

Protein Variants	Comment	Organism
additional information	aasS::kan insertion mutant retains low levels of acyl-acyl carrier protein synthetase	Vibrio harveyi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60400	-	SDS-PAGE, hexahistidine-tagged	Vibrio harveyi

Organism

Organism	UniProt	Comment	Textmining
Vibrio harveyi	Q00IB3	-	-
Vibrio harveyi B392	Q00IB3	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Vibrio harveyi

Storage Stability

Storage Stability	Organism
-80°C, 20 mM Tris-HCl (pH 7.5), 10% glycerol, 1 mM EDTA, 0.1 mM dithiothreitol and 0.002% Triton X-100, several months, no detectable loss of activity	Vibrio harveyi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + an acid + [acyl-carrier protein]	-	Vibrio harveyi	AMP + diphosphate + acyl-[acyl-carrier protein]	-	?
ATP + an acid + [acyl-carrier protein]	-	Vibrio harveyi B392	AMP + diphosphate + acyl-[acyl-carrier protein]	-	?
ATP + octanoate + [acyl-carrier protein]	acyl-carrier protein from Eschrichia coli is a good substrate, whilst octanoylation of the Lactococcus lactis acyl-carrier protein is very weak and that of the bovine mitochondrial acyl-carrier protein only barely detectable	Vibrio harveyi	AMP + diphosphate + octanoyl-[acyl-carrier protein]	-	?
ATP + octanoate + [acyl-carrier protein]	acyl-carrier protein from Eschrichia coli is a good substrate, whilst octanoylation of the Lactococcus lactis acyl-carrier protein is very weak and that of the bovine mitochondrial acyl-carrier protein only barely detectable	Vibrio harveyi B392	AMP + diphosphate + octanoyl-[acyl-carrier protein]	-	?
ATP + tetradecanoate + [acyl-carrier protein]	-	Vibrio harveyi	AMP + diphosphate + tetradecanoyl-[acyl-carrier protein]	-	?
ATP + tetradecanoate + [acyl-carrier protein]	-	Vibrio harveyi B392	AMP + diphosphate + tetradecanoyl-[acyl-carrier protein]	-	?

Synonyms

Synonyms	Comment	Organism
AasS	-	Vibrio harveyi
Acyl-acyl carrier protein synthetase	-	Vibrio harveyi

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Release 2023.1 (January 2023)