BRENDA - Enzyme Database
show all sequences of 6.2.1.18

Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in different lineages within the phylum Aquificae: Evidence for two ways of citrate cleavage

Hugler, M.; Huber, H.; Molyneaux, S.J.; Vetriani, C.; Sievert, S.M.; Environ. Microbiol. 9, 81-92 (2007)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aquifex aeolicus
-
-
-
Hydrogenobacter hydrogenophilus
-
-
-
Thermocrinis ruber
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.071
-
in 100 mM HEPES/NaOH, pH 8.3, 5 mM dithioerythritol, 5 mM MgCl2, 3 mM ATP, 0.5 mM CoA, 0.4 mM NADH, and 3 mM D-citrate
Aquifex aeolicus
0.082
-
in 100 mM HEPES/NaOH, pH 8.3, 5 mM dithioerythritol, 5 mM MgCl2, 3 mM ATP, 0.5 mM CoA, 0.4 mM NADH, and 3 mM D-citrate
Thermocrinis ruber
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + citrate + CoA
-
673369
Aquifex aeolicus
ADP + phosphate + citryl-CoA
-
-
-
?
ATP + citrate + CoA
-
673369
Thermocrinis ruber
ADP + phosphate + citryl-CoA
-
-
-
?
ATP + citrate + CoA
-
673369
Hydrogenobacter hydrogenophilus
ADP + phosphate + citryl-CoA
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Aquifex aeolicus
ATP
-
Hydrogenobacter hydrogenophilus
ATP
-
Thermocrinis ruber
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Aquifex aeolicus
ATP
-
Hydrogenobacter hydrogenophilus
ATP
-
Thermocrinis ruber
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.071
-
in 100 mM HEPES/NaOH, pH 8.3, 5 mM dithioerythritol, 5 mM MgCl2, 3 mM ATP, 0.5 mM CoA, 0.4 mM NADH, and 3 mM D-citrate
Aquifex aeolicus
0.082
-
in 100 mM HEPES/NaOH, pH 8.3, 5 mM dithioerythritol, 5 mM MgCl2, 3 mM ATP, 0.5 mM CoA, 0.4 mM NADH, and 3 mM D-citrate
Thermocrinis ruber
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + citrate + CoA
-
673369
Aquifex aeolicus
ADP + phosphate + citryl-CoA
-
-
-
?
ATP + citrate + CoA
-
673369
Thermocrinis ruber
ADP + phosphate + citryl-CoA
-
-
-
?
ATP + citrate + CoA
-
673369
Hydrogenobacter hydrogenophilus
ADP + phosphate + citryl-CoA
-
-
-
?
Other publictions for EC 6.2.1.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727577
Takacs-Vesbach
Metagenome sequence analysis o ...
Hydrogenobacter thermophilus
Front. Microbiol.
4
84
2013
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1
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10
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673369
Hugler
Autotrophic CO2 fixation via t ...
Aquifex aeolicus, Hydrogenobacter hydrogenophilus, Thermocrinis ruber
Environ. Microbiol.
9
81-92
2007
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Lill
Isolation of enzymically activ ...
Rattus norvegicus
Eur. J. Biochem.
125
645-650
1982
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