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Literature summary for 6.2.1.17 extracted from
- Propionyl-coenzyme A synthase from Chloroflexus aurantiacus, a key enzyme of the 3-hydroxypropionate cycle for autotrophic CO2 fixation (2002), J. Biol. Chem., 277, 12137-12143.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
NADPH | pH 7.8, 55°C | Chloroflexus aurantiacus |  |
| 0.01 | - |
CoA | pH 7.8, 55°C | Chloroflexus aurantiacus | |
| 0.015 | - |
3-hydroxypropionate | pH 7.8, 55°C | Chloroflexus aurantiacus | |
| 0.05 | - |
ATP | pH 7.8, 55°C | Chloroflexus aurantiacus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cs+ | can partially substitute for K+ | Chloroflexus aurantiacus | |
| K+ | required, half aximal activity at 4 mM | Chloroflexus aurantiacus | |
| Mg2+ | required | Chloroflexus aurantiacus | |
| NH4+ | can partially substitute for K+ | Chloroflexus aurantiacus | |
| Rb+ | can partially substitute for K+ | Chloroflexus aurantiacus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 185000 | - |
SDS-PAGE | Chloroflexus aurantiacus |
| 500000 | 800000 | gel filtration | Chloroflexus aurantiacus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-hydroxypropionate + CoA + NADPH | Chloroflexus aurantiacus | involved in CO2 fixation via 3-hydroxypropionate cycle | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
| ATP + 3-hydroxypropionate + CoA + NADPH | Chloroflexus aurantiacus OK-70-fl / DSM 636 | involved in CO2 fixation via 3-hydroxypropionate cycle | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chloroflexus aurantiacus | Q8VRG6 | - |
- |
| Chloroflexus aurantiacus OK-70-fl / DSM 636 | Q8VRG6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chloroflexus aurantiacus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
purified enzyme, pH 7.8, 55°C | Chloroflexus aurantiacus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% glycerol, 1 mM dithioerythreitol, stable for several weeks | Chloroflexus aurantiacus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-hydroxypropionate + CoA + NADPH | involved in CO2 fixation via 3-hydroxypropionate cycle | Chloroflexus aurantiacus | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
| ATP + 3-hydroxypropionate + CoA + NADPH | three-step reaction with 3-hydroxypropionyl-CoA and acryly-CoA as intermediates | Chloroflexus aurantiacus | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
| ATP + 3-hydroxypropionate + CoA + NADPH | involved in CO2 fixation via 3-hydroxypropionate cycle | Chloroflexus aurantiacus OK-70-fl / DSM 636 | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
| ATP + 3-hydroxypropionate + CoA + NADPH | three-step reaction with 3-hydroxypropionyl-CoA and acryly-CoA as intermediates | Chloroflexus aurantiacus OK-70-fl / DSM 636 | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
| ATP + acrylate + CoA + NADPH | same activity as with 3-hydroxypropionate | Chloroflexus aurantiacus | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
| ATP + acrylate + CoA + NADPH | same activity as with 3-hydroxypropionate | Chloroflexus aurantiacus OK-70-fl / DSM 636 | AMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
| GTP + 3-hydroxypropionate + CoA + NADPH | 24% of activity | Chloroflexus aurantiacus | GMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
| GTP + 3-hydroxypropionate + CoA + NADPH | 24% of activity | Chloroflexus aurantiacus OK-70-fl / DSM 636 | GMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
| additional information | no activity with glycolate, malonate, beta-alanine, 3-hydroxybutyrate, or crotonate | Chloroflexus aurantiacus | ? | - |
? | |
| additional information | no activity with glycolate, malonate, beta-alanine, 3-hydroxybutyrate, or crotonate | Chloroflexus aurantiacus OK-70-fl / DSM 636 | ? | - |
? | |
| UTP + 3-hydroxypropionate + CoA + NADPH | 20% of activity | Chloroflexus aurantiacus | UMP + diphosphate + NADP+ + propionyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | homotrimer or homotetramer | Chloroflexus aurantiacus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable for at least 15 min in presence of 20% glycerol and 1 mM dithioerythreitol | Chloroflexus aurantiacus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
3-hydroxypropionate | pH 7.8, 55°C | Chloroflexus aurantiacus | |
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