Literature summary for 6.2.1.16 extracted from

Hasegawa, S.; Imai, M.; Yamasaki, M.; Takahashi, N.; Fukui, T.
Transcriptional regulation of acetoacetyl-CoA synthetase by Sp1 in neuroblastoma cells (2018), Biochem. Biophys. Res. Commun., 495, 652-658 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene AACS, analysis of the transcriptional mechanism of AACS in Neuro-2a neuroblastoma cells. The minimal core promoter of the mouse AACS gene is located in a region with 110 bps upstream from the transcription start site. Mutagenesis studies show that the Sp1 binding site is crucial for AACS promoter activity. Sp1 binds to the Sp1 binding site on the promoter region of AACS. Overexpression of Sp1 increases AACS mRNA levels. Real-time PCR enzyme expression analysis. Functional analysis of the 5'-flanking region of AACS in Neuro-2a cells	Homo sapiens

Cloned (Comment)

Organism

gene AACS, analysis of the transcriptional mechanism of AACS in Neuro-2a neuroblastoma cells. The minimal core promoter of the mouse AACS gene is located in a region with 110 bps upstream from the transcription start site. Mutagenesis studies show that the Sp1 binding site is crucial for AACS promoter activity. Sp1 binds to the Sp1 binding site on the promoter region of AACS. Overexpression of Sp1 increases AACS mRNA levels. Real-time PCR enzyme expression analysis. Functional analysis of the 5'-flanking region of AACS in Neuro-2a cells

Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + acetoacetate + CoA	Homo sapiens	-	AMP + diphosphate + acetoacetyl-CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q86V21	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
Neuro-2a cell	-	Homo sapiens	-
neuroblastoma cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + acetoacetate + CoA	-	Homo sapiens	AMP + diphosphate + acetoacetyl-CoA	-	?

Synonyms

Synonyms	Comment	Organism
AACS	-	Homo sapiens
Acetoacetyl-CoA synthetase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	specificity protein 1 (Sp1) regulates gene expression of AACS in Neuro-2a cells, investigation of promoter activity of AACS, overview	additional information
Homo sapiens	specificity protein 1 (Sp1) binds to the Sp1 binding site on the promoter region of AACS. Overexpression of Sp1 increases AACS mRNA levels	up

General Information

General Information	Comment	Organism
malfunction	knockdown of SREBP-2, which orchestrates cholesterol synthesis, results in the downregulation of AACS mRNA levels. Knockdown of AACS results in a decrease in histone deacetylase 9, associated with gene silencing	Homo sapiens
physiological function	acetoacetyl-CoA synthetase (AACS) is the enzyme responsible for cholesterol and fatty acid synthesis in the cytosol. AACS has an important role in normal neuronal development. Specificity protein 1 (Sp1) regulates gene expression of AACS in Neuro-2a cells and ketone body utilization affects the balance of histone acetylation	Homo sapiens