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Literature summary for 6.2.1.16 extracted from

  • Fukui, T.; Ito, M.; Tomita, K.
    Purification and characterization of acetoacetyl-CoA synthetase from Zoogloea ramigera 1-16-M (1982), Eur. J. Biochem., 127, 423-428.
    View publication on PubMed
Search for more literature for 6.2.1.16

General Stability

General Stability Organism
in presence of 10% glycerol the enzyme can be frozen and thawed ten times without loss of activity Zoogloea ramigera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 ATP
-
 Zoogloea ramigera
0.076
-
 acetoacetate
-
 Zoogloea ramigera
0.091
-
 CoA
-
 Zoogloea ramigera
1.4
-
 L(+)-3-hydroxybutyrate
-
 Zoogloea ramigera

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can replace Mg2+ in activation, 26% of the activation relative to Mg2+ Zoogloea ramigera
Cs+ can replace K+ in activation Zoogloea ramigera
K+ monovalent cation required Zoogloea ramigera
Mg2+
-
 Zoogloea ramigera
Mn2+ can replace Mg2+ in activation Zoogloea ramigera
Mn2+ 96% of the activation relative to Mg2+ Zoogloea ramigera
additional information absolute requirement for a monovalent cation and a divalent cation Zoogloea ramigera
NH4+ can replace K+ in activation Zoogloea ramigera
Ni2+ can replace Mg2+ in activation Zoogloea ramigera
Ni2+ 21% of the activation relative to Mg2+ Zoogloea ramigera
Rb+ can replace K+ in activation Zoogloea ramigera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 gel filtration Zoogloea ramigera

Organism

Organism UniProt Comment Textmining
Zoogloea ramigera
-
-
-
Zoogloea ramigera 1-16-M / ATCC 19623
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Zoogloea ramigera

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52.2
-
-
 Zoogloea ramigera

Storage Stability

Storage Stability Organism
-20°C, 0.07 mg/ml enzyme, 10 mM Tris/HCl buffer, pH 7.5, 50% glycerol, 10 mM 2-mercaptoethanol, stable for at least 1 month Zoogloea ramigera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetoacetate + CoA r Zoogloea ramigera AMP + diphosphate + acetoacetyl-CoA
-
 ?
ATP + acetoacetate + CoA no activity with GTP, UTP, CTP or ADP Zoogloea ramigera AMP + diphosphate + acetoacetyl-CoA
-
 ?
ATP + acetoacetate + CoA r Zoogloea ramigera 1-16-M / ATCC 19623 AMP + diphosphate + acetoacetyl-CoA
-
 ?
ATP + acetoacetate + CoA no activity with GTP, UTP, CTP or ADP Zoogloea ramigera 1-16-M / ATCC 19623 AMP + diphosphate + acetoacetyl-CoA
-
 ?
ATP + L-3-hydroxybutanoate + CoA about 15% of the activity relative to ATP Zoogloea ramigera AMP + diphosphate + L-3-hydroxybutyryl-CoA
-
 ?
ATP + L-3-hydroxybutanoate + CoA about 15% of the activity relative to ATP Zoogloea ramigera 1-16-M / ATCC 19623 AMP + diphosphate + L-3-hydroxybutyryl-CoA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 72000, SDS-PAGE Zoogloea ramigera

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 1 d, 30% loss of activity, in absence of glycerol Zoogloea ramigera
30
-
 1 d, 70% loss of activity, in absence of glycerol Zoogloea ramigera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 formation of acetoacetate Zoogloea ramigera
8.4
-
 activation of acetoacetate Zoogloea ramigera