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Literature summary for 6.2.1.14 extracted from
- Pimelic acid, the first precursor of the Bacillus subtilis biotin synthesis pathway, exists as the free acid and is assembled by fatty acid synthesis (2017), Mol. Microbiol., 104, 595-607 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene bioW, encoded in the bio gene cluster, recombinant expression in and complementation of Escherichia coli DELTAbioC DELTA bioH mutant strain STL25, a derivative of strain MG1655 | Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | deletion of the chromosomal bioW through single crossover recombination by integration of recombinant vector pMUTIN4 blocks growth in biotin-free minimal media, growth phenotypes of Bacillus subtilis bioW and bioI mutant strains, overview. Expression of bioW from the Phyper-spank promoter of vector pDR111 inserted at an ectopic site (the amyE locus) restores growth only when promoter activity is induced with IPTG, biotin auxotrophy due to bioW inactivation. Bacillus subtilis strain BI274 has an engineered bio operon driven by a phage SP01 promoter resulting in overproduction of biotin | Bacillus subtilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Bacillus subtilis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 6-carboxyhexanoate + CoA | Bacillus subtilis | - |
AMP + diphosphate + 6-carboxyhexanoyl-CoA | - |
? | |
| ATP + 6-carboxyhexanoate + CoA | Bacillus subtilis 168 | - |
AMP + diphosphate + 6-carboxyhexanoyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P53559 | - |
- |
| Bacillus subtilis 168 | P53559 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | Bacillus subtilis cannot grow on acetate as sole carbon source | Bacillus subtilis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 6-carboxyhexanoate + CoA | - |
Bacillus subtilis | AMP + diphosphate + 6-carboxyhexanoyl-CoA | - |
? | |
| ATP + 6-carboxyhexanoate + CoA | - |
Bacillus subtilis 168 | AMP + diphosphate + 6-carboxyhexanoyl-CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BioW | - |
Bacillus subtilis |
| Pimeloyl-CoA synthetase | - |
Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bacillus subtilis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the biotin pathway genes responsible for pimelate moiety synthesis vary widely among bacteria whereas the ring synthesis genes are highly conserved. 6-Carboxyhexanoate-CoA ligase is essential in Bacillus subtilis, encoded by gene bioW, while it is not in Escherchia coli | Bacillus subtilis |
| malfunction | deletion of bioW causes a biotin auxotrophic phenotype whereas deletion of bioI does not. Growth phenotypes of Bacillus subtilis bioW and bioI mutant strains, biotin auxotrophy due to bioW inactivation, overview | Bacillus subtilis |
| metabolism | the enzyme catalyzes the first committed step of biotin biosynthesis, overview. The biotin pathway genes responsible for pimelate moiety synthesis vary widely among bacteria whereas the ring synthesis genes are highly conserved. Bacillus subtilis seems to have redundant genes, bioI and bioW, for generation of the pimelate intermediate. Pimelic acid originating from fatty acid synthesis pathway is a bona fide precursor of biotin in Bacillus subtilis. Synthesis of pimelate depends on fatty acid synthesis in Bacillus subtilis | Bacillus subtilis |
| physiological function | BioW is a pimeloyl-CoA synthetase that converts pimelic acid to pimeloyl-CoA. The essentiality of BioW for biotin synthesis indicates that the free form of pimelic acid is an intermediate in biotin synthesis. Bacillus subtilis has redundant genes, bioI and bioW, for generation of the pimelate intermediate. Expression of either Bacillus subtilis BioW or BioI bypasses the biotin auxotrophy of an Escherichia coli DELTAbioC DELTAbioH mutant strain SLT25 | Bacillus subtilis |
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