Cloned (Comment) | Organism |
---|---|
expressed in Nicotiana tabacum roots | Populus tomentosa |
Protein Variants | Comment | Organism |
---|---|---|
V388del | deletion of V338 residues in isoform 4CL1 results in activity towards sinapate | Populus tomentosa |
Y236F | the mutant of isoform 4CL2 exhibits high substrate catalytic efficiency for 4-coumarate but very low substrate affinities and specificities for caffeate and ferulate | Populus tomentosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01271 | - |
4-coumarate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
0.02611 | - |
4-coumarate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
0.02631 | - |
caffeate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
0.02643 | - |
caffeate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
0.02699 | - |
ferulate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
0.0272 | - |
4-coumarate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
0.03516 | - |
ferulate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
0.04217 | - |
ferulate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
0.04414 | - |
caffeate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
0.05399 | - |
4-coumarate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
0.05679 | - |
4-coumarate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
0.06132 | - |
caffeate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
0.06868 | - |
ferulate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
0.07366 | - |
cinnamate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
0.08261 | - |
ferulate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
0.08417 | - |
caffeate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
0.13571 | - |
cinnamate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
0.14266 | - |
cinnamate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
0.19649 | - |
cinnamate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
0.20002 | - |
cinnamate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Populus tomentosa | - |
AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tomentosa | H9AZ21 | - |
- |
Populus tomentosa | H9AZ22 | - |
- |
Populus tomentosa | H9AZ23 | - |
- |
Populus tomentosa | H9AZ24 | - |
- |
Populus tomentosa | Q941M3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bud | - |
Populus tomentosa | - |
leaf | - |
Populus tomentosa | - |
leaf | highest expression of isoform 4CL2 | Populus tomentosa | - |
leaf | highest expression of isoform 4CL4 | Populus tomentosa | - |
additional information | isoform 4CL1 is not expressed in bud and phloem | Populus tomentosa | - |
additional information | isoform 4CL2 is not expressed in xylem and root | Populus tomentosa | - |
additional information | isoform 4CL4 is not expressed in phloem | Populus tomentosa | - |
additional information | isoform 4CL5 is not expressed in phloem | Populus tomentosa | - |
phloem | - |
Populus tomentosa | - |
root | - |
Populus tomentosa | - |
root | highest expression of isoform 4CL1 | Populus tomentosa | - |
root | highest expression of isoform 4CL5 | Populus tomentosa | - |
xylem | - |
Populus tomentosa | - |
xylem | highest expression of isoform 4CL3 | Populus tomentosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Populus tomentosa | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Populus tomentosa | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + cinnamate + CoA | - |
Populus tomentosa | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + ferulate + CoA | - |
Populus tomentosa | AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + ferulate + CoA | good substrate for isoform 4CL4 | Populus tomentosa | AMP + diphosphate + feruloyl-CoA | - |
? | |
ATP + ferulate + CoA | good substrate for isoform 4CL5 | Populus tomentosa | AMP + diphosphate + feruloyl-CoA | - |
? | |
additional information | no activity with sinapate | Populus tomentosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-coumarate/coenzyme A ligase | - |
Populus tomentosa |
4CL1 | isoform | Populus tomentosa |
4CL2 | isoform | Populus tomentosa |
4CL3 | isoform | Populus tomentosa |
4CL4 | isoform | Populus tomentosa |
4CL5 | isoform | Populus tomentosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
cinnamate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
0.61 | - |
cinnamate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
0.96 | - |
ferulate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
1.17 | - |
cinnamate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
1.43 | - |
caffeate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
1.51 | - |
cinnamate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
1.66 | - |
caffeate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
2.23 | - |
ferulate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
3.22 | - |
cinnamate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
3.71 | - |
4-coumarate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
4.19 | - |
ferulate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
4.24 | - |
4-coumarate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
4.55 | - |
4-coumarate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
5.81 | - |
caffeate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
5.84 | - |
caffeate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
6.07 | - |
ferulate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
6.84 | - |
4-coumarate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
31.4 | - |
caffeate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
88.68 | - |
4-coumarate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
126.47 | - |
ferulate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Populus tomentosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.45 | - |
cinnamate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
5.95 | - |
cinnamate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
8.28 | - |
cinnamate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
11.13 | - |
cinnamate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
13.98 | - |
ferulate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
16.1 | - |
cinnamate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
23.32 | - |
caffeate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
52.88 | - |
ferulate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
62.81 | - |
caffeate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
69.38 | - |
caffeate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
73.48 | - |
ferulate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
74.66 | - |
4-coumarate | isoform 4CL1, pH and temperature not specified in the publication | Populus tomentosa | |
119.17 | - |
ferulate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
126.69 | - |
4-coumarate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
136.4 | - |
4-coumarate | isoform 4CL5, pH and temperature not specified in the publication | Populus tomentosa | |
220.83 | - |
caffeate | isoform 4CL3, pH and temperature not specified in the publication | Populus tomentosa | |
357.99 | - |
4-coumarate | isoform 4CL2, pH and temperature not specified in the publication | Populus tomentosa | |
711.37 | - |
caffeate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
3396.4 | - |
4-coumarate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa | |
4685.81 | - |
ferulate | isoform 4CL4, pH and temperature not specified in the publication | Populus tomentosa |