Cloned (Comment) | Organism |
---|---|
expression of GST-tagged isozyme Sa4CL1 in Escherichia coli | Sorbus aucuparia |
expression of His6-tagged isozyme Sa4CL2 in Escherichia coli | Sorbus aucuparia |
expression of His6-tagged isozyme Sa4CL3 in Escherichia coli | Sorbus aucuparia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state MichaelisMenten kinetics | Sorbus aucuparia | |
0.0009 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
0.0009 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
0.0025 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
0.0054 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
0.0068 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
0.0073 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
0.009 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
0.0125 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
0.021 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
0.023 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
0.033 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
0.05 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme Sa4CL3 contains a 49-amino acid N-terminal extension, which includes a chloroplast sorting signal | Sorbus aucuparia | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can substitute Mg2+ | Sorbus aucuparia | |
Mg2+ | required | Sorbus aucuparia | |
Mn2+ | can substitute Mg2+ | Sorbus aucuparia | |
additional information | strict dependence of Sa4CLs on a divalent cation, no activity with Cu2+ | Sorbus aucuparia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59500 | - |
x * 59500, about, isozyme Sa4CL1, sequence calculation | Sorbus aucuparia |
60100 | - |
x * 60100, about, isozyme Sa4CL2, sequence calculation | Sorbus aucuparia |
66200 | - |
x * 66200, about, isozyme Sa4CL3, sequence calculation | Sorbus aucuparia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Sorbus aucuparia | - |
AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + cinnamate + CoA | Sorbus aucuparia | - |
AMP + diphosphate + cinnamoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorbus aucuparia | D5LLN7 | isozyme Sa4CL1; isozyme Sa4CL1 | - |
Sorbus aucuparia | D5LNB5 | isozyme Sa4CL2; isozyme Sa4CL2 | - |
Sorbus aucuparia | D5LNB6 | isozyme Sa4CL3; isozyme Sa4CL3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Sorbus aucuparia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Sorbus aucuparia | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | preferred substrate | Sorbus aucuparia | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Sorbus aucuparia | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + cinnamate + CoA | - |
Sorbus aucuparia | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
ATP + ferulate + CoA | - |
Sorbus aucuparia | AMP + diphosphate + ferulyl-CoA | - |
? | |
additional information | no activity with benzoate and sinapate | Sorbus aucuparia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59500, about, isozyme Sa4CL1, sequence calculation | Sorbus aucuparia |
? | x * 60100, about, isozyme Sa4CL2, sequence calculation | Sorbus aucuparia |
? | x * 66200, about, isozyme Sa4CL3, sequence calculation | Sorbus aucuparia |
Synonyms | Comment | Organism |
---|---|---|
4-coumarate:CoA ligase | - |
Sorbus aucuparia |
Sa4CL1 | - |
Sorbus aucuparia |
Sa4CL2 | - |
Sorbus aucuparia |
Sa4CL3 | - |
Sorbus aucuparia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Sorbus aucuparia |
37 | - |
- |
Sorbus aucuparia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
0.3 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
0.34 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
0.36 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
0.37 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
0.38 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
0.49 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
0.58 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
0.78 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
0.8 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
0.96 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
1 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Sorbus aucuparia |
7.5 | - |
- |
Sorbus aucuparia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sorbus aucuparia |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sorbus aucuparia | sequence calculation | - |
5.5 |
Sorbus aucuparia | sequence calculation | - |
5.6 |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetically, isozymes Sa4CL1 and Sa4CL2 belong to the class I cluster and Sa4CL3 groups in the class II cluster | Sorbus aucuparia |
metabolism | the enzyme is involved in the flavonoid biosynthesis from cinnamic acid, it is the last enzyme of the general phenylpropanoid pathway, overview | Sorbus aucuparia |
physiological function | Sorbus aucuparia cell cultures accumulate biphenyl and dibenzofuran phytoalexins from the starter substrate benzoyl-CoA in response to elicitor treatment involving cinnamate:CoA ligase and benzoate:CoA ligase, but not 4-coumarate:CoA ligase | Sorbus aucuparia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
10 | - |
cinnamate | pH 7.5, 22°C | Sorbus aucuparia | |
60 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
90 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
120 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
120 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia | |
130 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
230 | - |
4-coumarate | pH 7.5, 22°C | Sorbus aucuparia | |
360 | - |
ferulate | pH 7.5, 22°C | Sorbus aucuparia | |
410 | - |
caffeate | pH 7.5, 22°C | Sorbus aucuparia |