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Literature summary for 6.2.1.12 extracted from
- Identification of a 4-coumarate:CoA ligase gene family in the moss, Physcomitrella patens (2008), Phytochemistry, 69, 2449-2456.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Physcomitrium patens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
4-coumarate | recombinant isozyme 4CL1 | Physcomitrium patens |  |
| 0.016 | - |
4-coumarate | recombinant isozyme 4CL2 | Physcomitrium patens | |
| 0.025 | - |
caffeate | recombinant isozyme 4CL1 | Physcomitrium patens | |
| 0.029 | - |
4-coumarate | recombinant isozyme 4CL3 | Physcomitrium patens | |
| 0.045 | - |
caffeate | recombinant isozyme 4CL2 | Physcomitrium patens | |
| 0.05 | - |
cinnamate | recombinant isozyme 4CL1 | Physcomitrium patens | |
| 0.064 | - |
cinnamate | recombinant isozyme 4CL3 | Physcomitrium patens | |
| 0.066 | - |
cinnamate | recombinant isozyme 4CL2 | Physcomitrium patens | |
| 0.073 | - |
ferulate | recombinant isozyme 4CL2 | Physcomitrium patens | |
| 0.187 | - |
ferulate | recombinant isozyme 4CL1 | Physcomitrium patens | |
| 0.725 | - |
caffeate | recombinant isozyme 4CL3 | Physcomitrium patens | |
| 0.8 | - |
ferulate | recombinant isozyme 4CL3 | Physcomitrium patens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
SDS-PAGE, isozyme 4CL3 | Physcomitrium patens |
| 65000 | - |
SDS-PAGE, isozyme 4CL1 | Physcomitrium patens |
| 65000 | - |
SDS-PAGE, isozyme 4CL2 | Physcomitrium patens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Physcomitrium patens | B7SB99 | isozyme 4CL1; subspecies Physcomitrella patens patens | - |
| Physcomitrium patens | B7SBA0 | isozyme 4CL2; subspecies Physcomitrella patens patens | - |
| Physcomitrium patens | B7SBA1 | isozyme 4CL3; subspecies Physcomitrella patens patens | - |
| Physcomitrium patens | B7SBA2 | isozyme 4CL4; subspecies Physcomitrella patens patens | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Physcomitrium patens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gametophore | - |
Physcomitrium patens | - |
| protonema | - |
Physcomitrium patens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | highest catalytic efficiency towards 4-coumarate | Physcomitrium patens | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + caffeate + CoA | - |
Physcomitrium patens | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + cinnamate + CoA | - |
Physcomitrium patens | AMP + diphosphate + cinnamoyl-CoA | - |
ir | |
| ATP + ferulate + CoA | - |
Physcomitrium patens | AMP + diphosphate + feruloyl-CoA | - |
? | |
| additional information | sinapate is not accepted as a substrate | Physcomitrium patens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, isozyme 4CL1, SDS-PAGE | Physcomitrium patens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-coumarate:CoA ligase | - |
Physcomitrium patens |
| 4CL1 | isozyme | Physcomitrium patens |
| 4CL2 | isozyme | Physcomitrium patens |
| 4CL3 | isozyme | Physcomitrium patens |
| 4CL4 | isozyme | Physcomitrium patens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Physcomitrium patens | |
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