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Literature summary for 6.2.1.12 extracted from

  • Lindermayr, C.; Mollers, B.; Fliegmann, J.; Uhlmann, A.; Lottspeich, F.; Meimberg, H.; Ebel, J.
    Divergent members of a soybean (Glycine max L.) 4-coumarate:coenzyme A ligase gene family (2002), Eur. J. Biochem., 269, 1304-1315.
    View publication on PubMed
Search for more literature for 6.2.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as His-tag fusion protein Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 ferulate
-
 Glycine max
0.009
-
 4-coumarate
-
 Glycine max
0.01
-
 4-coumarate
-
 Glycine max
0.011
-
 sinapate
-
 Glycine max
0.013
-
 caffeate
-
 Glycine max
0.022
-
 4-coumarate
-
 Glycine max
0.033
-
 caffeate
-
 Glycine max
0.034
-
 caffeate
-
 Glycine max
0.042
-
 4-coumarate
-
 Glycine max
0.05
-
 caffeate
-
 Glycine max
0.083
-
 3,4-dimethoxycinnamate
-
 Glycine max
0.14
-
 ferulate
-
 Glycine max
0.26
-
 cinnamate
-
 Glycine max
1.1
-
 cinnamate
-
 Glycine max
1.3
-
 ferulate
-
 Glycine max
1.7
-
 cinnamate
-
 Glycine max
3.1
-
 ferulate
-
 Glycine max
4.4
-
 cinnamate
-
 Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-coumarate + CoA Glycine max plant phenylpropanoid metabolism AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max P31687 4CL4, cDNA; four isoforms 4CL1-4
-
Glycine max Q8S564 4CL1, cDNA; four isoforms 4CL1-4
-
Glycine max Q8S5C1 4CL2, cDNA; four isoforms 4CL1-4
-
Glycine max Q8S5C2 4CL3, genomic DNA; four isoforms 4CL1-4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3,4-dimethoxycinnamate + CoA
-
 Glycine max AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA
-
 ?
ATP + 4-coumarate + CoA
-
 Glycine max AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + 4-coumarate + CoA plant phenylpropanoid metabolism Glycine max AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
ATP + caffeate + CoA
-
 Glycine max AMP + diphosphate + caffeoyl-CoA
-
 ?
ATP + cinnamate + CoA
-
 Glycine max AMP + diphosphate + cinnamoyl-CoA
-
 ?
ATP + ferulate + CoA
-
 Glycine max AMP + diphosphate + feruloyl-CoA
-
 ?
ATP + sinapate + CoA
-
 Glycine max AMP + diphosphate + sinapoyl-CoA
-
 ?
additional information no reaction with 3,4-dimethoxycinnamate and sinapate Glycine max ?
-
 ?