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Literature summary for 6.2.1.12 extracted from
- Isoenzymes of hydroxycinnamate:CoA ligase from poplar stems. Properties and tissue distribution (1983), Planta, 158, 225-229.
General Stability
| General Stability | Organism |
|---|---|
| 30% glycerol or 15% ethylene glycol stabilizes | Populus x canadensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
4-coumarate | isoenzyme II | Populus x canadensis |  |
| 0.011 | - |
ferulate | isoenzyme II | Populus x canadensis | |
| 0.013 | - |
4-coumarate | isoenzyme III | Populus x canadensis | |
| 0.015 | - |
ferulate | isoenzyme III | Populus x canadensis | |
| 0.017 | - |
4-coumarate | 5-hydroxyferulate | Populus x canadensis | |
| 0.02 | - |
ferulate | ferulate, , isoenzyme I | Populus x canadensis | |
| 0.022 | - |
sinapoate | isoenzyme I | Populus x canadensis | |
| 0.042 | - |
4-coumarate | isoenzyme I | Populus x canadensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
gel filtration | Populus x canadensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | Populus x canadensis | the isoenzymes can play an important role in the control of the monomeric composition of lignins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus x canadensis | - |
3 isoenzymes: I, II, III | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Populus x canadensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| parenchyma | 3 isoenzymes are present at very low level of activity, isoenzyme II is the major isoenzyme | Populus x canadensis | - |
| sclerenchyma | only isoenzyme I is detectable | Populus x canadensis | - |
| stem | - |
Populus x canadensis | - |
| xylem | 3 isoenzymes present | Populus x canadensis | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| stable for several weeks at -20°C or two days at 0°C, in 0.1 M Tris-HCl buffer, pH 7.5, 30% glycerol or 15% ethylene glycol | Populus x canadensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | - |
Populus x canadensis | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
| ATP + 4-coumarate + CoA | the isoenzymes can play an important role in the control of the monomeric composition of lignins | Populus x canadensis | ? | - |
? | |
| ATP + 5-hydroxyferulate + CoA | isoenzyme I is active, no activity is detectable with isoenzyme II and III | Populus x canadensis | AMP + diphosphate + 5-hydroxyferuloyl-CoA | - |
? | |
| ATP + caffeate + CoA | isoenzyme III is active, no activity is detectable with isoenzyme I and II | Populus x canadensis | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + ferulate + CoA | - |
Populus x canadensis | AMP + diphosphate + feruloyl-CoA | - |
? | |
| ATP + sinapoate + CoA | isoenzyme I is active, no activity with isoenzyme II and III | Populus x canadensis | AMP + diphosphate + sinapoyl-CoA | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8 | Tris-HCl or sodium-potassium phosphate buffer | Populus x canadensis |
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