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Literature summary for 6.2.1.12 extracted from
- Enzymic synthesis of lignin precursors. Purification and properties of 4-coumarate:CoA ligase from cambial sap of spruce (Picia abies L.) (1982), Eur. J. Biochem., 123, 583-586.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
gel filtration | Picea abies |
| 63000 | - |
1 * 63000, SDS-PAGE | Picea abies |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Picea abies | - |
isoenzymes not detected | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Picea abies |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cambium | sap | Picea abies | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Picea abies |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM sodium/potassium phosphate, pH 7.0, 42 mM mercaptoethanol, 20% ethyleneglycol, stable for several months | Picea abies |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4-coumarate + CoA | 94% of the activity relative to ferulate | Picea abies | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |  |
| ATP + 5-hydroxyferulate + CoA | 18% of the activity relative to ferulate | Picea abies | AMP + diphosphate + 5-hydroxyferuloyl-CoA | - |
? | |
| ATP + caffeate + CoA | 86% of the activity relative to ferulate | Picea abies | AMP + diphosphate + caffeoyl-CoA | - |
? | |
| ATP + cinnamate + CoA | 14% of the activity relative to ferulate | Picea abies | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
| ATP + ferulate + CoA | - |
Picea abies | AMP + diphosphate + feruloyl-CoA | - |
? | |
| ATP + sinapoate + CoA | no activity | Picea abies | AMP + diphosphate + sinapoyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 63000, SDS-PAGE | Picea abies |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Picea abies |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.5 | 6.8: about 80% of maximal activity, 7.5: maximal activity | Picea abies |
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Release 2023.1 (January 2023)
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