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Literature summary for 6.2.1.11 extracted from

  • Tanaka, M.; Yamamoto H.; Izumi, Y.; Yamada, H.
    Purification and properties of biotinyl-CoA synthetase from Mycoplana sp. No. 166 (1986), Arch. Biochem. Biophys., 251, 479-486.
    View publication on PubMed
Search for more literature for 6.2.1.11

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Mycoplana sp.
2,2'-dipyridyl
-
 Mycoplana sp.
Cu2+
-
 Mycoplana sp.
EDTA
-
 Mycoplana sp.
Fe3+
-
 Mycoplana sp.
Hg2+
-
 Mycoplana sp.
iodoacetic acid
-
 Mycoplana sp.
Ni2+
-
 Mycoplana sp.
p-chloromercuribenzoate
-
 Mycoplana sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 CoA
-
 Mycoplana sp.
0.3
-
 (+/-)-dethiobiotin
-
 Mycoplana sp.
0.32
-
 (+)-biotin
-
 Mycoplana sp.
0.43
-
 DL-actithiazic acid
-
 Mycoplana sp.
2.8
-
 ATP
-
 Mycoplana sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycoplana sp.
Mn2+ can replace Mg2+ Mycoplana sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 gel filtration Mycoplana sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + biotin + CoA Mycoplana sp. first enzyme involved in biotin degradation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycoplana sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycoplana sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mycoplana sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (+/-)-dethiobiotin + CoA
-
 Mycoplana sp. AMP + diphosphate + dethiobiotinyl-CoA
-
 ?
ATP + 5-(2-thienyl)-n-valerate + CoA 19% of the activity relative to biotin Mycoplana sp. AMP + diphosphate + 5-(2-thienyl)-n-valeryl-CoA
-
 ?
ATP + actithiazic acid + CoA 59% of the activity relative to biotin Mycoplana sp. AMP + diphosphate + actithiazyl-CoA
-
 ?
ATP + alpha-dehydrobiotin + CoA 27% of the activity relative to biotin Mycoplana sp. AMP + diphosphate + alpha-dehydrobiotinyl-CoA
-
 ?
ATP + biotin + CoA
-
 Mycoplana sp. AMP + diphosphate + biotinyl-CoA
-
 ?
ATP + biotin + CoA first enzyme involved in biotin degradation Mycoplana sp. ?
-
 ?
ATP + dethiobiotin + CoA 52% of the activity relative to biotin Mycoplana sp. AMP + diphosphate + dethiobiotinyl-CoA
-
 ?
ATP + n-caprylate + CoA 19% of the activity relative to biotin Mycoplana sp. AMP + diphosphate + n-capryloyl-CoA
-
 ?
ATP + n-decanoate + CoA 14% of the activity relative to biotin Mycoplana sp. AMP + diphosphate + n-decanoyl-CoA
-
 ?
ATP + n-laurate + CoA 11% of the activity relative to biotin Mycoplana sp. AMP + diphosphate + n-lauroyl-CoA
-
 ?

Subunits

Subunits Comment Organism
monomer SDS-PAGE Mycoplana sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Mycoplana sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 35 20 min, stable Mycoplana sp.
25
-
 pH 6.5-8.5, 1 h, stable Mycoplana sp.
35
-
 20 min, pH 7.4, stable Mycoplana sp.
40
-
 20 min, pH 7.4, 54% loss of activity Mycoplana sp.
45
-
 20 min, pH 7.4, 85% loss of activity Mycoplana sp.
50
-
 20 min, pH 7.4, 93% loss of activity Mycoplana sp.
60
-
 20 min, pH 7.4, 96% loss of activity Mycoplana sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Mycoplana sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 25°C, 1 h, stable Mycoplana sp.