Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.1 extracted from

  • VanDrisse, C.M.; Escalante-Semerena, J.C.
    In Streptomyces lividans, acetyl-CoA synthetase activity is controlled by O-serine and Nepsilon-lysine acetylation (2018), Mol. Microbiol., 107, 577-594 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Streptomyces lividans

Protein Variants

Protein Variants Comment Organism
S608T when mutant enzyme S608T is acetylated, it loses about 80% activity compared to the wild type Streptomyces lividans

Inhibitors

Inhibitors Comment Organism Structure
additional information when the enzyme is acetylated, it loses about 20% activity compared to the non-acetylated control Streptomyces lividans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate + CoA Streptomyces lividans
-
 AMP + diphosphate + acetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces lividans
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
acetylation
-
 Streptomyces lividans

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography, and Superdex 200 gel filtration Streptomyces lividans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA
-
 Streptomyces lividans AMP + diphosphate + acetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 72000, His6-tagged enzyme, SDS-PAGE Streptomyces lividans

Synonyms

Synonyms Comment Organism
Acetyl-CoA synthetase
-
 Streptomyces lividans
ACS
-
 Streptomyces lividans

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Streptomyces lividans