Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanothermobacter thermautotrophicus |
expression in Escherichia coli, AF-ACS2 | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
CoA | - |
Methanothermobacter thermautotrophicus | |
0.3 | - |
CoA | AF-ACS2 | Archaeoglobus fulgidus | |
1.7 | - |
acetate | AF-ACS2 | Archaeoglobus fulgidus | |
2.9 | - |
ATP | AF-ACS2 | Archaeoglobus fulgidus | |
3.3 | - |
ATP | - |
Methanothermobacter thermautotrophicus | |
3.5 | - |
acetate | - |
Methanothermobacter thermautotrophicus | |
3.9 | - |
propionate | AF-ACS2 | Archaeoglobus fulgidus | |
36.5 | - |
propionate | - |
Methanothermobacter thermautotrophicus | |
133 | - |
Butyrate | AF-ACS2 | Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 30% of the activation with Mg2+ or Mn2+ | Methanothermobacter thermautotrophicus | |
Ca2+ | about 70% of the activation with Mg2+ or Mn2+, AF-ACS2 | Archaeoglobus fulgidus | |
Co2+ | about 70% of the activation with Mg2+ or Mn2+, AF-ACS2 | Archaeoglobus fulgidus | |
Co2+ | about 80% of the activation with Mg2+ or Mn2+ | Methanothermobacter thermautotrophicus | |
Mg2+ | AF-ACS2 shows strong preference for Mg2+ and Mn2+ as the divalent metal | Archaeoglobus fulgidus | |
Mg2+ | strong preference for Mg2+ and Mn2+ as the divalent metal | Methanothermobacter thermautotrophicus | |
Mn2+ | AF-ACS2 shows strong preference for Mg2+ and Mn2+ as the divalent metal | Archaeoglobus fulgidus | |
Mn2+ | strong preference for Mg2+ and Mn2+ as the divalent metal | Methanothermobacter thermautotrophicus | |
Ni2+ | about 25% of the activation with Mg2+ or Mn2+ | Methanothermobacter thermautotrophicus | |
Ni2+ | about 35% of the activation with Mg2+ or Mn2+, AF-ACS2 | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71556 | - |
2 * 71556, calculated from sequence | Methanothermobacter thermautotrophicus |
77587 | - |
3 * 77587, AF-ACS2, calculated from sequence | Archaeoglobus fulgidus |
144800 | - |
gel filtration | Methanothermobacter thermautotrophicus |
221200 | - |
AF-ACS2, gel filtration | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | Archaeoglobus fulgidus | acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | Methanothermobacter thermautotrophicus | acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA | AMP + diphosphate + acetyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Methanothermobacter thermautotrophicus | Q2XNL6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
AF-ACS2 | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetate + CoA | acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA | Archaeoglobus fulgidus | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA | Methanothermobacter thermautotrophicus | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | AF-ACS2 has 2.3fold higher affinity and catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed | Archaeoglobus fulgidus | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + acetate + CoA | MT-ACS1 is limited to acetate and propionate as acyl substrates. MT-ACS1 has nearly 11fold higher affinity and 14fold higher catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed | Methanothermobacter thermautotrophicus | AMP + diphosphate + acetyl-CoA | - |
? | |
ATP + butyrate + CoA | AF-ACS2 | Archaeoglobus fulgidus | AMP + diphosphate + butyryl-CoA | - |
? | |
ATP + propionate + CoA | AF-ACS2 has 2.3fold higher affinity and catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed | Archaeoglobus fulgidus | AMP + diphosphate + propionyl-CoA | - |
? | |
ATP + propionate + CoA | MT-ACS1 is limited to acetate and propionate as acyl substrates. MT-ACS1 has nearly 11fold higher affinity and 14fold higher catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed | Methanothermobacter thermautotrophicus | AMP + diphosphate + propionyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 71556, calculated from sequence | Methanothermobacter thermautotrophicus |
trimer | 3 * 77587, AF-ACS2, calculated from sequence | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
acetate:CoA ligase (AMP-forming) | - |
Archaeoglobus fulgidus |
acetate:CoA ligase (AMP-forming) | - |
Methanothermobacter thermautotrophicus |
adenosine monophosphate-forming acetyl-CoA synthetase | - |
Archaeoglobus fulgidus |
adenosine monophosphate-forming acetyl-CoA synthetase | - |
Methanothermobacter thermautotrophicus |
AF-ACS2 | - |
Archaeoglobus fulgidus |
AMP-forming acetyl-CoA synthetase | - |
Archaeoglobus fulgidus |
AMP-forming acetyl-CoA synthetase | - |
Methanothermobacter thermautotrophicus |
MT-ACS1 | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 70 | - |
Methanothermobacter thermautotrophicus |
65 | 70 | AF-ACS2 | Archaeoglobus fulgidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 85 | 45°C: about 55% of maximal activity, 85°C: about 60% of maximal activity, AF-ACS2 | Archaeoglobus fulgidus |
45 | 75 | 45°C: about 55% of maximal activity, 75°C: about 50% of maximal activity | Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
Butyrate | AF-ACS2 | Archaeoglobus fulgidus | |
0.7 | - |
ATP | - |
Methanothermobacter thermautotrophicus | |
9.1 | - |
propionate | AF-ACS2 | Archaeoglobus fulgidus | |
13.2 | - |
ATP | AF-ACS2 | Archaeoglobus fulgidus | |
21.2 | - |
acetate | AF-ACS2 | Archaeoglobus fulgidus | |
46.3 | - |
propionate | - |
Methanothermobacter thermautotrophicus | |
65.4 | - |
acetate | - |
Methanothermobacter thermautotrophicus | |
66.6 | - |
ATP | - |
Methanothermobacter thermautotrophicus | |
81.6 | - |
CoA | - |
Methanothermobacter thermautotrophicus | |
144.9 | - |
CoA | AF-ACS2 | Archaeoglobus fulgidus |