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Literature summary for 6.2.1.1 extracted from

  • Ingram-Smith, C.; Smith, K.S.
    AMP-forming acetyl-CoA synthetases in Archaea show unexpected diversity in substrate utilization (2007), Archaea, 2, 95-107.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanothermobacter thermautotrophicus
expression in Escherichia coli, AF-ACS2 Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
CoA
-
Methanothermobacter thermautotrophicus
0.3
-
CoA AF-ACS2 Archaeoglobus fulgidus
1.7
-
acetate AF-ACS2 Archaeoglobus fulgidus
2.9
-
ATP AF-ACS2 Archaeoglobus fulgidus
3.3
-
ATP
-
Methanothermobacter thermautotrophicus
3.5
-
acetate
-
Methanothermobacter thermautotrophicus
3.9
-
propionate AF-ACS2 Archaeoglobus fulgidus
36.5
-
propionate
-
Methanothermobacter thermautotrophicus
133
-
Butyrate AF-ACS2 Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 30% of the activation with Mg2+ or Mn2+ Methanothermobacter thermautotrophicus
Ca2+ about 70% of the activation with Mg2+ or Mn2+, AF-ACS2 Archaeoglobus fulgidus
Co2+ about 70% of the activation with Mg2+ or Mn2+, AF-ACS2 Archaeoglobus fulgidus
Co2+ about 80% of the activation with Mg2+ or Mn2+ Methanothermobacter thermautotrophicus
Mg2+ AF-ACS2 shows strong preference for Mg2+ and Mn2+ as the divalent metal Archaeoglobus fulgidus
Mg2+ strong preference for Mg2+ and Mn2+ as the divalent metal Methanothermobacter thermautotrophicus
Mn2+ AF-ACS2 shows strong preference for Mg2+ and Mn2+ as the divalent metal Archaeoglobus fulgidus
Mn2+ strong preference for Mg2+ and Mn2+ as the divalent metal Methanothermobacter thermautotrophicus
Ni2+ about 25% of the activation with Mg2+ or Mn2+ Methanothermobacter thermautotrophicus
Ni2+ about 35% of the activation with Mg2+ or Mn2+, AF-ACS2 Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71556
-
2 * 71556, calculated from sequence Methanothermobacter thermautotrophicus
77587
-
3 * 77587, AF-ACS2, calculated from sequence Archaeoglobus fulgidus
144800
-
gel filtration Methanothermobacter thermautotrophicus
221200
-
AF-ACS2, gel filtration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate + CoA Archaeoglobus fulgidus acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA AMP + diphosphate + acetyl-CoA
-
?
ATP + acetate + CoA Methanothermobacter thermautotrophicus acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA AMP + diphosphate + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-
Methanothermobacter thermautotrophicus Q2XNL6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus
AF-ACS2 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA Archaeoglobus fulgidus AMP + diphosphate + acetyl-CoA
-
?
ATP + acetate + CoA acetate-CoA ligase is a key enzyme for conversion of acetate to acetyl-CoA Methanothermobacter thermautotrophicus AMP + diphosphate + acetyl-CoA
-
?
ATP + acetate + CoA AF-ACS2 has 2.3fold higher affinity and catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed Archaeoglobus fulgidus AMP + diphosphate + acetyl-CoA
-
?
ATP + acetate + CoA MT-ACS1 is limited to acetate and propionate as acyl substrates. MT-ACS1 has nearly 11fold higher affinity and 14fold higher catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed Methanothermobacter thermautotrophicus AMP + diphosphate + acetyl-CoA
-
?
ATP + butyrate + CoA AF-ACS2 Archaeoglobus fulgidus AMP + diphosphate + butyryl-CoA
-
?
ATP + propionate + CoA AF-ACS2 has 2.3fold higher affinity and catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed Archaeoglobus fulgidus AMP + diphosphate + propionyl-CoA
-
?
ATP + propionate + CoA MT-ACS1 is limited to acetate and propionate as acyl substrates. MT-ACS1 has nearly 11fold higher affinity and 14fold higher catalytic efficiency with acetate than with propionate. Enzyme shows a strong preference for ATP versus CTP, GTP, TTP, UTP, ITP or ADP, for which less than 5% activity is observed Methanothermobacter thermautotrophicus AMP + diphosphate + propionyl-CoA
-
?

Subunits

Subunits Comment Organism
dimer 2 * 71556, calculated from sequence Methanothermobacter thermautotrophicus
trimer 3 * 77587, AF-ACS2, calculated from sequence Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
acetate:CoA ligase (AMP-forming)
-
Archaeoglobus fulgidus
acetate:CoA ligase (AMP-forming)
-
Methanothermobacter thermautotrophicus
adenosine monophosphate-forming acetyl-CoA synthetase
-
Archaeoglobus fulgidus
adenosine monophosphate-forming acetyl-CoA synthetase
-
Methanothermobacter thermautotrophicus
AF-ACS2
-
Archaeoglobus fulgidus
AMP-forming acetyl-CoA synthetase
-
Archaeoglobus fulgidus
AMP-forming acetyl-CoA synthetase
-
Methanothermobacter thermautotrophicus
MT-ACS1
-
Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65 70
-
Methanothermobacter thermautotrophicus
65 70 AF-ACS2 Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 85 45°C: about 55% of maximal activity, 85°C: about 60% of maximal activity, AF-ACS2 Archaeoglobus fulgidus
45 75 45°C: about 55% of maximal activity, 75°C: about 50% of maximal activity Methanothermobacter thermautotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
Butyrate AF-ACS2 Archaeoglobus fulgidus
0.7
-
ATP
-
Methanothermobacter thermautotrophicus
9.1
-
propionate AF-ACS2 Archaeoglobus fulgidus
13.2
-
ATP AF-ACS2 Archaeoglobus fulgidus
21.2
-
acetate AF-ACS2 Archaeoglobus fulgidus
46.3
-
propionate
-
Methanothermobacter thermautotrophicus
65.4
-
acetate
-
Methanothermobacter thermautotrophicus
66.6
-
ATP
-
Methanothermobacter thermautotrophicus
81.6
-
CoA
-
Methanothermobacter thermautotrophicus
144.9
-
CoA AF-ACS2 Archaeoglobus fulgidus