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Literature summary for 6.2.1.1 extracted from

  • Londesborough, J.C.; Webster, L.T.
    Fatty acyl-CoA synthetases (1974), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 10, 469-488.
No PubMed abstract available
Search for more literature for 6.2.1.1

Activating Compound

Activating Compound Comment Organism Structure
acetate required for ATP-diphosphate exchange Bos taurus

General Stability

General Stability Organism
photoinactivation in presence of methylene blue Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
seleno-CoA competitive to CoA Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 ATP in Tris buffer, pH 6.7, 200 mM K+ Bos taurus
0.1
-
 ATP in Pipes buffer, pH 6.7, 200 mM K+ Bos taurus
0.14
-
 acetate pH 6.7, 200 mM K+ Bos taurus
0.21
-
 CoA
-
 Bos taurus
0.22
-
 acetate CoA, in Tris buffer, pH 8.3, 200 mM K+ Bos taurus
0.28
-
 CoA in Tris buffer, pH 6.7, 200 mM K+ Bos taurus
0.35
-
 ATP Tris buffer, pH 8.3, 200 mM K+ Bos taurus
0.65
-
 acetate
-
 Bos taurus
0.71
-
 ATP
-
 Bos taurus
1.2
-
 CoA in Pipes buffer, pH 6.7, 200 mM K+ Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ two types of divalent metal ion requirement, 1. Mg2+, Mn2+, Fe2+, Co2+ or Ca2+ required for the formation of the enzyme-bound acetyl adenylate, 2. Ni2+, Cd2+, Fe2+ or Cu2+ required for adenylate binding Bos taurus
Cd2+ two types of divalent metal ion requirement, 1. Mg2+, Mn2+, Fe2+, Co2+ or Ca2+ required for the formation of the enzyme-bound acetyl adenylate, 2. Ni2+, Cd2+, Fe2+ or Cu2+ required for adenylate binding Bos taurus
Co2+ two types of divalent metal ion requirement, 1. Mg2+, Mn2+, Fe2+, Co2+ or Ca2+ required for the formation of the enzyme-bound acetyl adenylate, 2. Ni2+, Cd2+, Fe2+ or Cu2+ required for adenylate binding Bos taurus
Cu2+ two types of divalent metal ion requirement, 1. Mg2+, Mn2+, Fe2+, Co2+ or Ca2+ required for the formation of the enzyme-bound acetyl adenylate, 2. Ni2+, Cd2+, Fe2+ or Cu2+ required for adenylate binding Bos taurus
Fe2+ two types of divalent metal ion requirement, 1. Mg2+, Mn2+, Fe2+, Co2+ or Ca2+ required for the formation of the enzyme-bound acetyl adenylate, 2. Ni2+, Cd2+, Fe2+ or Cu2+ required for adenylate binding Bos taurus
K+ activates, absolute requirement for certain monovalent cations, no inhibition at high concentrations Bos taurus
Li+ activation at 5-8 mM, absolute requirement for certain monovalent cations, inhibition above 10 mM Bos taurus
Mg2+ required Bos taurus
Mg2+ two types of divalent metal ion requirement, 1. Mg2+, Mn2+, Fe2+, Co2+ or Ca2+ required for the formation of the enzyme-bound acetyl adenylate, 2. Ni2+, Cd2+, Fe2+ or Cu2+ required for adenylate binding Bos taurus
Mn2+ two types of divalent metal ion requirement, 1. Mg2+, Mn2+, Fe2+, Co2+ or Ca2+ required for the formation of the enzyme-bound acetyl adenylate, 2. Ni2+, Cd2+, Fe2+ or Cu2+ required for adenylate binding Bos taurus
Na+ activation at 5-8 mM, absolute requirement for certain monovalent cations, inhibition above 10 mM Bos taurus
NH4+ activates, absolute requirement for certain monovalent cations, no inhibition at high concentrations Bos taurus
Ni2+ two types of divalent metal ion requirement, 1. Mg2+, Mn2+, Fe2+, Co2+ or Ca2+ required for the formation of the enzyme-bound acetyl adenylate, 2. Ni2+, Cd2+, Fe2+ or Cu2+ required for adenylate binding Bos taurus
Rb+ activates, absolute requirement for certain monovalent cations, no inhibition at high concentrations Bos taurus
Tris activates, absolute requirement for certain monovalent cations, no inhibition at high concentrations Bos taurus

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Bos taurus
-
-
-
Euglena gracilis
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue
-
 Rattus norvegicus
-
brain
-
 Rattus norvegicus
-
brain
-
 Bos taurus
-
heart
-
 Rattus norvegicus
-
heart
-
 Bos taurus
-
liver
-
 Rattus norvegicus
-
liver
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA
-
 Rattus norvegicus AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA
-
 Oryctolagus cuniculus AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA
-
 Euglena gracilis AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA
-
 Aspergillus niger AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + CoA neither glutathione nor pantetheine can substitute for CoA as acyl acceptor Bos taurus AMP + diphosphate + acetyl-CoA
-
 ?
ATP + acetate + seleno-CoA
-
 Bos taurus AMP + diphosphate + acetyl-seleno-CoA
-
 ?
ATP + acrylate + CoA
-
 Bos taurus AMP + diphosphate + acryloyl-CoA
-
 ?
ATP + butyrate + CoA isobutyrate Bos taurus AMP + diphosphate + butyryl-CoA
-
 ?
ATP + fluoroacetate + CoA
-
 Bos taurus AMP + diphosphate + fluoroacetyl-CoA
-
 ?
ATP + propanoate + CoA
-
 Bos taurus AMP + diphosphate + propanoyl-CoA
-
 ?
dATP + acetate + CoA 70% of the activity relative to ATP Bos taurus dAMP + diphosphate + acetyl-CoA
-
 ?
additional information
-
 Oryctolagus cuniculus ?
-
 ?
additional information enzyme catalyzes acetate-dependent ATP-diphosphate exchange Bos taurus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 ATP-diphosphate exchange Bos taurus