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Literature summary for 6.2.1.1 extracted from
- Acetyl-CoA synthetase from baker¦s yeast (Saccharomyces cerevisiae) (1981), Methods Enzymol., 71, 317-324.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bicarbonate | - |
Saccharomyces cerevisiae |  |
| Cl- | - |
Saccharomyces cerevisiae | |
| monovalent cations | at 200 mM | Saccharomyces cerevisiae | |
| SO42- | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
CoA | - |
Saccharomyces cerevisiae | |
| 0.28 | - |
acetate | - |
Saccharomyces cerevisiae | |
| 1.2 | - |
ATP | - |
Saccharomyces cerevisiae | |
| 10 | - |
propanoate | - |
Saccharomyces cerevisiae | |
| 17 | - |
Acrylate | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae | |
| Mg2+ | Km: 4.0 mM | Saccharomyces cerevisiae | |
| Mn2+ | 10 mM, 38% of the activity relative to Mg2+. Progressive inactivation of the enzyme by MnCl2 is not reversible by subsequent addition of MgCl2 | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
x * 78000, SDS-PAGE | Saccharomyces cerevisiae |
| 151000 | - |
sedimentation equilibrium analysis | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA | ordered bi uni uni bi ping-pong mechanism with ordered substrate addition and release | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.5 M potassium phosphate, pH 7.5, 7 mM 2-mercaptoethanol and 0.5 mM EDTA, protein concentration above 5 mg/ml, no loss of activity after 6 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate + CoA | - |
Saccharomyces cerevisiae | AMP + diphosphate + acetyl-CoA | - |
? | |
| ATP + acrylate + CoA | - |
Saccharomyces cerevisiae | AMP + diphosphate + acryloyl-CoA | - |
? | |
| ATP + propanoate + CoA | - |
Saccharomyces cerevisiae | AMP + diphosphate + propanoyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78000, SDS-PAGE | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.8 | about 50% of maximal activity at pH 6.8 and 8.8 | Saccharomyces cerevisiae |
| 7.3 | 8.1 | 90% of maximal activity at pH 7.3 and 8.1 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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