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Literature summary for 6.2.1.1 extracted from

  • Maruyama, K.
    Short-chain acyl-coenzyme A synthetase in Rhodopseudomonas sphaeroides (1982), J. Biochem., 91, 725-730.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
acetate
-
Cereibacter sphaeroides
3.1
-
propanoate
-
Cereibacter sphaeroides
5.26
-
Acrylate
-
Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, can replace Mg2+ in activation, with 30% of the efficiency relative to Mg2+ Cereibacter sphaeroides
Mg2+ required Cereibacter sphaeroides
Mn2+ 10 mM, 90% of the activation relative to Mg2+ Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-20°C, 0.2 mM DTT, stable for at least one month Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate + CoA
-
Cereibacter sphaeroides AMP + diphosphate + acetyl-CoA
-
?
ATP + acrylate + CoA
-
Cereibacter sphaeroides AMP + diphosphate + acryloyl-CoA
-
?
ATP + propanoate + CoA
-
Cereibacter sphaeroides AMP + diphosphate + propanoyl-CoA
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Cereibacter sphaeroides