Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
determination of strucure by molecular replacement, to 1.5 A resolution. Enzyme possesses a Zn2+ located in the putative tRNA acceptor stem-binding domain. The Zn2+ ion is coordinated by three cysteine and a tyrosine ligand | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutamol-adenosine monophosphate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
ATP | pH 7.2, 37°C, activation of L-glutamate | Escherichia coli | |
6 | - |
L-glutamate | pH 7.2, 37°C, activation of L-glutamate | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | enzyme possesses a Zn2+ ion located in the putative tRNA acceptor stem-binding domain. The Zn2+ ion is coordinated by three cysteine and a tyrosine ligand | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P27305 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNA | acceptor is unfractionated RNA | Escherichia coli | AMP + diphosphate + L-glutamyl-tRNA | the activation step of L-glutamate is tRNA-independent | ? | |
additional information | enzyme does not catalyse attachment of the activated L-glutamate to Escherichia coli tRNAGlu | Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
L-glutamate | pH 7.2, 37°C, activation of L-glutamate | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
glutamol-adenosine monophosphate | pH 7.2, 37°C, substrate ATP, activation of L-glutamate | Escherichia coli | |
0.44 | - |
glutamol-adenosine monophosphate | pH 7.2, 37°C, substrate L-glutamate, activation of L-glutamate | Escherichia coli |