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Literature summary for 6.1.1.9 extracted from

  • Tardif, K.D.; Horowitz, J.
    Functional group recognition at the aminoacylation and editing sites of E. coli valyl-tRNA synthetase (2004), RNA, 10, 493-503.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 L-valine with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C Escherichia coli
0.0043
-
 L-valine with wild-type tRNAVal, pH 7.5, 37°C Escherichia coli
0.0048
-
 L-valine with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C Escherichia coli
0.005
-
 L-valine with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C Escherichia coli
0.0088
-
 L-valine with mutant tRNAVal with 3'-terminal purine riboside base analogue, pH 7.5, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-valine + tRNAVal Escherichia coli
-
 AMP + diphosphate + L-valyl-tRNAVal
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 catalytic activity with L-valine and diverse tRNA analogues with functional group substitutions at the terminal nucleoside of the 3'-end, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-valine + tRNAVal
-
 Escherichia coli AMP + diphosphate + L-valyl-tRNAVal
-
 ?
ATP + L-valine + tRNAVal,3' 2-aminopurine mutant tRNAVal with 3'-terminal 2-aminopurine base analogue substitution Escherichia coli AMP + diphosphate + L-valyl-tRNAVal, 3' 2-aminopurine
-
 ?
ATP + L-valine + tRNAVal,3' inosine mutant tRNAVal with 3'-terminal inosine base analogue substitution Escherichia coli AMP + diphosphate + L-valyl-tRNAVal, 3' inosine
-
 ?
ATP + L-valine + tRNAVal,3' isoguanosine mutant tRNAVal with 3'-terminal isoguanosine base analogue substitution Escherichia coli AMP + diphosphate + L-valyl-tRNAVal, 3' isoguanosine
-
 ?
ATP + L-valine + tRNAVal,3' purine riboside mutant tRNAVal with 3'-terminal purine riboside base analogue substitution Escherichia coli AMP + diphosphate + L-valyl-tRNAVal, 3' purine riboside
-
 ?
additional information catalytic activity with L-valine and diverse tRNA analogues with functional group substitutions at the terminal nucleoside of the 3'-end, some of which stimulate the editing reaction, overview Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
G7a
-
 Escherichia coli
Synthetase, valyl-transfer ribonucleate
-
 Escherichia coli
Valine transfer ribonucleate ligase
-
 Escherichia coli
Valine translase
-
 Escherichia coli
Valine--tRNA ligase
-
 Escherichia coli
ValRS
-
 Escherichia coli
Valyl transfer ribonucleic acid synthetase
-
 Escherichia coli
Valyl-transfer ribonucleate synthetase
-
 Escherichia coli
Valyl-transfer RNA synthetase
-
 Escherichia coli
Valyl-tRNA ligase
-
 Escherichia coli
Valyl-tRNA synthetase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.68
-
 L-valine with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C Escherichia coli
0.99
-
 L-valine with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C Escherichia coli
1.06
-
 L-valine with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C Escherichia coli
6.08
-
 L-valine with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C Escherichia coli
6.08
-
 L-valine with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C Escherichia coli
6.08
-
 L-valine with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C Escherichia coli
13.9
-
 L-valine with wild-type tRNAVal, pH 7.5, 37°C Escherichia coli
14.4
-
 L-valine with mutant tRNAVal with 3'-terminal purine riboside base analogue, pH 7.5, 37°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli