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Literature summary for 6.1.1.9 extracted from

  • Kakitani, M.; Tonomura, B.; Hiromi, K.
    Valyl-tRNA synthetase from Bacillus stearothermophilus. Purification and binding with the substrates L-valine and ATP (1986), Agric. Biol. Chem., 50, 2437-2444.
No PubMed abstract available
Search for more literature for 6.1.1.9

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 1 * 100000, SDS-PAGE Geobacillus stearothermophilus
110000
-
 gel filtration Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
 NCA 1503
-
Geobacillus stearothermophilus NCA 1503
-
 NCA 1503
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0045
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-valine + tRNAVal
-
 Geobacillus stearothermophilus AMP + diphosphate + L-valyl-tRNAVal
-
 ?
ATP + L-valine + tRNAVal
-
 Geobacillus stearothermophilus NCA 1503 AMP + diphosphate + L-valyl-tRNAVal
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
-
 Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 52°C, aminoacylation Geobacillus stearothermophilus
8.5
-
 30E, aminoacylation Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 6.5: 30-40% of maximal activity, 10: 10% of maximal activity Geobacillus stearothermophilus