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Literature summary for 6.1.1.9 extracted from

  • Godar, D.E.; Yang, D.C.H.
    Mammalian high molecular weight and monomeric forms of valyl-tRNA synthetase (1988), Biochemistry, 27, 2181-2186.
    View publication on PubMed

General Stability

General Stability Organism
bovine serum albumin protects from thermal inactivation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
KCl 50% inhibition at 100 mM, optimal activation at 25 mM Rattus norvegicus
Mg2+ 50% inhibition at 7 mM, optimal activation at 4 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
tRNAVal tetrameric enzyme form Rattus norvegicus
0.00092
-
tRNAVal monomeric enzyme form Rattus norvegicus
0.0082
-
valine monomeric enzyme form Rattus norvegicus
0.01
-
valine tetrameric enzyme form Rattus norvegicus
0.029
-
ATP tetrameric enzyme form Rattus norvegicus
0.071
-
ATP monomeric enzyme form Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimal concentration: 25 mM Rattus norvegicus
KCl 50% inhibition at 100 mM Rattus norvegicus
Mg2+ 50% inhibition at 7 mM Rattus norvegicus
Mg2+ optimal concentration: 4 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
1 * 140000, SDS-PAGE Rattus norvegicus
140000
-
4 * 140000, SDS-PAGE, the high molecular weight valyl-tRNA synthetase is a homotypic tetramer which converts to the monomeric valyl-tRNA synthetase after cleavage of a small peptide Rattus norvegicus
145000
-
sucrose density gradient centrifugation, monomeric enzyme form Rattus norvegicus
585000
-
gel filtration, tetrameric enzyme form Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.722
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-180°C, purified enzyme highly unstable, stored immediately after purification at -180°C Rattus norvegicus
-20°C, homogenate and ammonium sulfate fraction are stable for 6 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-valine + tRNAVal
-
Rattus norvegicus AMP + diphosphate + L-valyl-tRNAVal
-
?

Subunits

Subunits Comment Organism
monomer 1 * 140000, SDS-PAGE Rattus norvegicus
monomer the high molecular weight valyl-tRNA synthetase is a homotypic tetramer which converts to the monomeric valyl-tRNA synthetase after cleavage of a small peptide Rattus norvegicus
tetramer 4 * 140000, SDS-PAGE, the high molecular weight valyl-tRNA synthetase is a homotypic tetramer which converts to the monomeric valyl-tRNA synthetase after cleavage of a small peptide Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
no activity detectable at 60°C Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
half-life: 90 min Rattus norvegicus
20
-
half-life: 30 min, when enzyme is stabilized by 1 mg/ml bovine serum albumin and 50% glycerol Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 pH 6-7.5: optimum, pH 8.5: 50% of maximal activity Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7
-
Mg2+
-
Rattus norvegicus