General Stability | Organism |
---|---|
bovine serum albumin protects from thermal inactivation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 50% inhibition at 100 mM, optimal activation at 25 mM | Rattus norvegicus | |
Mg2+ | 50% inhibition at 7 mM, optimal activation at 4 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
tRNAVal | tetrameric enzyme form | Rattus norvegicus | |
0.00092 | - |
tRNAVal | monomeric enzyme form | Rattus norvegicus | |
0.0082 | - |
valine | monomeric enzyme form | Rattus norvegicus | |
0.01 | - |
valine | tetrameric enzyme form | Rattus norvegicus | |
0.029 | - |
ATP | tetrameric enzyme form | Rattus norvegicus | |
0.071 | - |
ATP | monomeric enzyme form | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal concentration: 25 mM | Rattus norvegicus | |
KCl | 50% inhibition at 100 mM | Rattus norvegicus | |
Mg2+ | 50% inhibition at 7 mM | Rattus norvegicus | |
Mg2+ | optimal concentration: 4 mM | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
1 * 140000, SDS-PAGE | Rattus norvegicus |
140000 | - |
4 * 140000, SDS-PAGE, the high molecular weight valyl-tRNA synthetase is a homotypic tetramer which converts to the monomeric valyl-tRNA synthetase after cleavage of a small peptide | Rattus norvegicus |
145000 | - |
sucrose density gradient centrifugation, monomeric enzyme form | Rattus norvegicus |
585000 | - |
gel filtration, tetrameric enzyme form | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.722 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-180°C, purified enzyme highly unstable, stored immediately after purification at -180°C | Rattus norvegicus |
-20°C, homogenate and ammonium sulfate fraction are stable for 6 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-valine + tRNAVal | - |
Rattus norvegicus | AMP + diphosphate + L-valyl-tRNAVal | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 140000, SDS-PAGE | Rattus norvegicus |
monomer | the high molecular weight valyl-tRNA synthetase is a homotypic tetramer which converts to the monomeric valyl-tRNA synthetase after cleavage of a small peptide | Rattus norvegicus |
tetramer | 4 * 140000, SDS-PAGE, the high molecular weight valyl-tRNA synthetase is a homotypic tetramer which converts to the monomeric valyl-tRNA synthetase after cleavage of a small peptide | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
no activity detectable at 60°C | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
half-life: 90 min | Rattus norvegicus |
20 | - |
half-life: 30 min, when enzyme is stabilized by 1 mg/ml bovine serum albumin and 50% glycerol | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6-7.5: optimum, pH 8.5: 50% of maximal activity | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
Mg2+ | - |
Rattus norvegicus |