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Literature summary for 6.1.1.9 extracted from

  • Colas, B.; Imbault, P.; Sarantoglou, V.; Boulanger, Y.; Weil, J.H.
    Chloroplastic and cytoplasmic valyl- and leucyl-tRNA synthetases from Euglena gracilis. Comparative study of their structural properties (1982), Biochim. Biophys. Acta, 697, 71-77.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Euglena gracilis 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
126000
-
1 * 126000, urea-SDS-PAGE Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Euglena gracilis

Storage Stability

Storage Stability Organism
4°C, 50 mM potassium phosphate buffer, pH 7.0, 1 mM MgCl2, 0.002 mM L-valine, 100 mM NaCl, 15% v/v 1,2-propanediol, stable for several weeks Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-valine + tRNAVal
-
Euglena gracilis AMP + diphosphate + L-valyl-tRNAVal
-
?

Subunits

Subunits Comment Organism
monomer 1 * 126000, urea-SDS-PAGE Euglena gracilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
5 min, chloroplastic enzyme loses 30% of its activity, mitochondrial enzyme 75% Euglena gracilis