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Literature summary for 6.1.1.7 extracted from

  • Filley, S.J.; Hill, K.A.W.
    Amino acid substitutions at position 73 in motif 2 of Escherichia coli alanyl-tRNA synthetase (1993), Arch. Biochem. Biophys., 307, 46-51.
    View publication on PubMed
Search for more literature for 6.1.1.7

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
L73A mutant enzymes with replacement of Lys73 with Gln, Asn, Ala or Glu show reduction in catalytic efficiency in aminoacylation assay. Glu substitution causes a 5-fold decrease in affinity for alanine Escherichia coli
L73E mutant enzymes with replacement of Lys73 with Gln, Asn, Ala or Glu show reduction in catalytic efficiency in aminoacylation assay. Glu substitution causes a 5-fold decrease in affinity for alanine Escherichia coli
L73N mutant enzymes with replacement of Lys73 with Gln, Asn, Ala or Glu show reduction in catalytic efficiency in aminoacylation assay. Glu substitution causes a 5-fold decrease in affinity for alanine Escherichia coli
L73Q mutant enzymes with replacement of Lys73 with Gln, Asn, Ala or Glu show reduction in catalytic efficiency in aminoacylation assay. Glu substitution causes a 5-fold decrease in affinity for alanine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 tRNAAla wild-type AlaRS, aminoacylation Escherichia coli
0.022
-
 tRNAAla mutant AlaRS(K73Q), aminoacylation Escherichia coli
0.023
-
 tRNA mutant AlaRS(K73E), aminoacylation Escherichia coli
0.028
-
 tRNAAla mutant AlaR(K73A), aminoacylation Escherichia coli
0.14
-
 ATP wild-type AlaRS and mutant AlaRS(K73E), AlaRS(K73Q), ATP-diphosphate exchange Escherichia coli
0.2
-
 ATP mutant AlaRS(K73A), ATP-diphosphate exchange Escherichia coli
0.3
-
 alanine wild-type AlaRS and mutant AlaRS(K73Q), ATP-diphosphate exchange Escherichia coli
0.31
-
 alanine mutant AlaRS(K73A), ATP-diphosphate exchange Escherichia coli
1.41
-
 alanine mutant AlaRS(K73E), ATP-diphosphate exchange Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 wild-type and mutant enzymes with replacement of Lys73 with Gln, Asn, Ala or Glu
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-alanine + tRNA
-
 Escherichia coli ?
-
 ?
ATP + L-alanine + tRNAAla
-
 Escherichia coli AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
additional information alanine dependent ATP-diphosphate exchange: alanine + ATP + enzyme /alanine-AMP-enzyme + diphosphate Escherichia coli ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
 tRNAAla mutant AlaRS(K73E), aminoacylation Escherichia coli
1.4
-
 tRNAAla mutant AlaRS(K73A), aminoacylation Escherichia coli
1.5
-
 tRNAAla AlaRS, aminoacylation Escherichia coli
1.7
-
 tRNAAla mutant AlaRS(K73Q), ATP-diphosphate exchange Escherichia coli
82
-
 ATP mutant AlaRS(K73Q), ATP-diphosphate exchange Escherichia coli
89
-
 ATP AlaRS, ATP-diphosphate exchange Escherichia coli
120
-
 ATP AlaRS(K73A), ATP-diphosphate exchange Escherichia coli