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Literature summary for 6.1.1.6 extracted from

  • Oka, M.; Takegawa, K.; Kimura, Y.
    Enzymatic characterization of a class II lysyl-tRNA synthetase, LysS, from Myxococcus xanthus (2015), Arch. Biochem. Biophys., 579, 33-39 .
    View publication on PubMed
Search for more literature for 6.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
gene lysS, recombinant expression of His-tagged enzyme in Escherichia coli Myxococcus xanthus

Inhibitors

Inhibitors Comment Organism Structure
ADP the addition of ADP inhibits the diadenosine tetraphosphate synthesis by Ap4A synthase activity of LysS. Ap4A production decreases by 22% and 52% at 1 mM and 5 mM ADP, respectively. In the presence of ADP, Ap3A is not synthesized by LysS during 30 min of incubation. ADP may act as a competitive inhibitor of LysS in the first step reaction, i.e. lysyl-AMP formation from lysine and ATP Myxococcus xanthus
Zn2+ inhibits enzyme activity in the presence of Mn2+ Myxococcus xanthus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Myxococcus xanthus
Mn2+ the enzyme requires Mn2+ for the synthesis of diadenosine tetraphosphate (Ap4A) Myxococcus xanthus
additional information Zn2+ is not required for diadenosine tetraphosphate (Ap4A) synthesis by LysS Myxococcus xanthus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 recombinant His-tagged enzyme, gel filtration Myxococcus xanthus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-lysine + tRNALys Myxococcus xanthus
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys Myxococcus xanthus DK 1622
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus Q1D380
-
-
Myxococcus xanthus DK 1622 Q1D380
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, dialysis, EDTA treatment, and again dialysis Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALys
-
 Myxococcus xanthus AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys
-
 Myxococcus xanthus DK 1622 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
additional information lysyl-tRNA synthetases efficiently produce diadenosine tetraphosphate (Ap4A) from lysyl-AMP with ATP in the absence of tRNA. When incubated with ATP, Mn2+, lysine, and inorganic diphosphatase, LysS first produces Ap4A and ADP, then converts Ap4A to diadenosine triphosphate (Ap3A), and finally converts Ap3A to ADP, the end product of the reaction. Recombinant LysS retains Ap4A synthase activity without lysine addition. When incubated with Ap4A (minus pyrophosphatase), LysS converts Ap4A mainly ATP and AMP, or ADP in the presence or absence of lysine, respectively. LysS converts Ap4A to yield ATP and AMP when Mn2+ and lysine are present, formation of ATP is higher than that of AMP. LysS hydrolyzes Ap4A symmetrically yielding ADP Myxococcus xanthus ?
-
 ?
additional information lysyl-tRNA synthetases efficiently produce diadenosine tetraphosphate (Ap4A) from lysyl-AMP with ATP in the absence of tRNA. When incubated with ATP, Mn2+, lysine, and inorganic diphosphatase, LysS first produces Ap4A and ADP, then converts Ap4A to diadenosine triphosphate (Ap3A), and finally converts Ap3A to ADP, the end product of the reaction. Recombinant LysS retains Ap4A synthase activity without lysine addition. When incubated with Ap4A (minus pyrophosphatase), LysS converts Ap4A mainly ATP and AMP, or ADP in the presence or absence of lysine, respectively. LysS converts Ap4A to yield ATP and AMP when Mn2+ and lysine are present, formation of ATP is higher than that of AMP. LysS hydrolyzes Ap4A symmetrically yielding ADP Myxococcus xanthus DK 1622 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 x 58000, about, recombinant His-tagged enzyme, sequence calculation, 1 * 60000, recombinant His-tagged enzyme, SDS-PAGE Myxococcus xanthus

Synonyms

Synonyms Comment Organism
Ap4A synthase
-
 Myxococcus xanthus
class II lysyl-tRNA synthetase
-
 Myxococcus xanthus
lysS
-
 Myxococcus xanthus
MXAN_4731
-
 Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Myxococcus xanthus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 the relative activity of LysS at 30-60°C is 49%, 77%, or 45%, respectively, compared to the activity at the optimal temperature of 50°C Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Myxococcus xanthus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 optimal activity at pH 8.0-9.0, 80% of maximal activity at pH 7.0 Myxococcus xanthus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Myxococcus xanthus

General Information

General Information Comment Organism
evolution the lysyl-tRNA synthetase of Mycobacterium smegmatis belongs to the class II amino acid tRNA synthetases Myxococcus xanthus
physiological function activity of most class II aminoacyl-tRNA synthetases absolutely requires the presence of added cognate amino acids Myxococcus xanthus