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Literature summary for 6.1.1.6 extracted from

  • Hoepfner, D.; McNamara, C.W.; Lim, C.S.; Studer, C.; Riedl, R.; Aust, T.; McCormack, S.L.; Plouffe, D.M.; Meister, S.; Schuierer, S.; Plikat, U.; Hartmann, N.; Staedtler, F.; Cotesta, S.; Schmitt, E.K.; Petersen, F.; Supek, F.; Glynne, R.J.; Tallarico, J.A.; Porter, J.A.; Fishman, M.C.; Bodenreider, C.; Di, D.i.a.
    Selective and specific inhibition of the Plasmodium falciparum lysyl-tRNA synthetase by the fungal secondary metabolite cladosporin (2012), Cell Host Microbe, 11, 654-663.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner (DE3) cells Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
cladosporin 30% inhibition at 0.11 mM Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-lysine + tRNALys Plasmodium falciparum
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys Plasmodium falciparum FCB
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys Plasmodium falciparum NF54
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys Plasmodium falciparum Camp R
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys Plasmodium falciparum FCR3
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys Plasmodium falciparum D1
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys Plasmodium falciparum D6
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-
Plasmodium falciparum Camp R
-
-
-
Plasmodium falciparum D1
-
-
-
Plasmodium falciparum D6
-
-
-
Plasmodium falciparum FCB
-
-
-
Plasmodium falciparum FCR3
-
-
-
Plasmodium falciparum NF54
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography, and MonoQ column chromatography Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALys
-
 Plasmodium falciparum AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys
-
 Plasmodium falciparum FCB AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys
-
 Plasmodium falciparum NF54 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys
-
 Plasmodium falciparum Camp R AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys
-
 Plasmodium falciparum FCR3 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys
-
 Plasmodium falciparum D1 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
ATP + L-lysine + tRNALys
-
 Plasmodium falciparum D6 AMP + diphosphate + L-lysyl-tRNALys
-
 ?

Synonyms

Synonyms Comment Organism
KRS
-
 Plasmodium falciparum
Lysyl-tRNA synthetase
-
 Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Plasmodium falciparum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000454
-
 in 40 mM HEPES (pH 7.5), at 22°C Plasmodium falciparum cladosporin
0.0000574
-
 in 40 mM HEPES (pH 7.5), at 22°C Plasmodium falciparum cladosporin
0.0000667
-
 in 40 mM HEPES (pH 7.5), at 22°C Plasmodium falciparum cladosporin
0.0000721
-
 in 40 mM HEPES (pH 7.5), at 22°C Plasmodium falciparum cladosporin
0.0000779
-
 in 40 mM HEPES (pH 7.5), at 22°C Plasmodium falciparum cladosporin
0.0000801
-
 in 40 mM HEPES (pH 7.5), at 22°C Plasmodium falciparum cladosporin
0.0000879
-
 in 40 mM HEPES (pH 7.5), at 22°C Plasmodium falciparum cladosporin
0.0000896
-
 in 40 mM HEPES (pH 7.5), at 22°C Plasmodium falciparum cladosporin