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Literature summary for 6.1.1.6 extracted from

  • Cen, S.; Javanbakht, H.; Niu, M.; Kleiman, L.
    Ability of wild-type and mutant lysyl-tRNA synthetase to facilitate tRNA(Lys) incorporation into human immunodeficiency virus type 1 (2004), J. Virol., 78, 1595-1601.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
COS7 cells are cotransfected with a plasmid containing HIV-1 proviral DNA and a plasmid containing cDNA coding for either full-length human LysRS or a truncated LysRS variant in which the N-terminal 65 amino acids have been deleted (DELTA1-65 LysRS). N-terminally truncated LysRS, which binds poorly to tRNALys, does not increase tRNALys packaging into viruses, while C-terminally truncated LysRS (DELTA452-597), which binds to but does not aminoacylate tRNALys, still facilitates an increase in tRNALys packaging into virions Homo sapiens

Protein Variants

Protein Variants Comment Organism
DELTA1-65 mutant enzyme binds poorly to tRNALys, but does not increase tRNALys packaging into HIV-1 viruses Homo sapiens
DELTA452-597 mutant enzyme binds to but does not aminoacylate tRNALys, still facilitates an increase in tRNALys packaging into virions Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALys
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Homo sapiens AMP + L-lysyl-tRNALys + diphosphate
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