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Literature summary for 6.1.1.6 extracted from

  • Terada, T.; Nureki, O.; Ishitani, R.; Ambrogelly, A.; Ibba, M.; Soll, D.; Yokoyama, S.
    Functional convergence of two lysyl-tRNA synthetases with unrelated topologies (2002), Nat. Struct. Biol., 9, 257-262.
    View publication on PubMed
Search for more literature for 6.1.1.6

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme alone or in complex with L-lysine, 0.001 ml of protein solution 5 mg/ml, with or without 10 mM L-lysine, with an equal volume of reservoir solution: 120 mM HEPES, pH 7.5, 2.4 M ammonium sulfate, 2.4% v/v PEG 400, 20°C, X-ray diffraction structure determination at 2.6 A for the enzyme crystals and at 2.9 A for the complex crystals, structure analysis Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-lysine + tRNALys Pyrococcus horikoshii
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O57963 class I enzyme, i.e. LysRS-I
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys mechanism of tRNALys and L-lysine binding Pyrococcus horikoshii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-lysine + tRNALys
-
 Pyrococcus horikoshii AMP + L-lysyl-tRNALys + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
L-Lysine-transfer RNA ligase
-
 Pyrococcus horikoshii
Lysine translase
-
 Pyrococcus horikoshii
Lysine--tRNA ligase
-
 Pyrococcus horikoshii
Lysine-tRNA synthetase
-
 Pyrococcus horikoshii
LysRS
-
 Pyrococcus horikoshii
LysRS-I
-
 Pyrococcus horikoshii
LysRS-II
-
 Pyrococcus horikoshii
Lysyl-transfer ribonucleate synthetase
-
 Pyrococcus horikoshii
Lysyl-transfer RNA synthetase
-
 Pyrococcus horikoshii
Lysyl-tRNA synthetase
-
 Pyrococcus horikoshii
More enzyme has structural similarity to glutamyl-tRNA synthetase, EC 6.1.1.17, from other organisms, e.g. Thermus thermophilus Pyrococcus horikoshii
Synthetase, lysyl-transfer ribonucleate
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Pyrococcus horikoshii