Cloned (Comment) | Organism |
---|---|
expression of the C-terminally His-tagged N-terminal extension of the enzyme in Escherichia coli strain BL21(DE3) | Cricetinae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | binding kinetics of acceptor stem and anticodon domains of the tRNALys | Cricetinae | |
0.002 | - |
yeast tRNALys | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
0.0028 | - |
yeast tRNALys | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
0.003 | - |
human tRNALys3 | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
0.0117 | - |
human tRNALys3 | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
0.092 | - |
L-lysine | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
0.104 | - |
L-lysine | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
0.195 | - |
ATP | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
0.25 | - |
ATP | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
8608 | - |
recombinant N-terminal extension, mass spectrometry | Cricetinae |
8741 | - |
recombinant N-terminal extension, DNA sequence determination | Cricetinae |
9104 | - |
recombinant N-terminal extension, centrifugation sedimentation equilibrium | Cricetinae |
24700 | - |
recombinant N-terminal extension, gel filtration | Cricetinae |
62000 | - |
2 * 62000, N-terminally truncated recombinant enzyme, SDS-PAGE | Cricetinae |
120000 | - |
N-terminally truncated recombinant enzyme, gel filtration | Cricetinae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + lysine + tRNALys | Cricetinae | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetinae | - |
purified recombinant enzyme expressed in Saccharomyces cerevisiae, wild-type and N-terminally truncated form, the latter prepared by proteolytic cleavage by elastase | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged N-terminal extension of the enzyme from expression in Escherichia coli, to homogeneity | Cricetinae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-lysine + human tRNALys3 | - |
Cricetinae | AMP + diphosphate + L-lysyl-tRNALys3 | - |
? | |
ATP + L-lysine + yeast tRNALys | - |
Cricetinae | AMP + diphosphate + L-lysyl-tRNALys | - |
? | |
ATP + lysine + tRNALys | - |
Cricetinae | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + lysine + tRNALys | tRNALys from Saccharomyces cerevisiae, recombinant N-terminal extension provides the enzyme with RNA binding properties, the N-terminally truncated enzyme is no longer able to bind the RNA substrate | Cricetinae | AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 62000, N-terminally truncated recombinant enzyme, SDS-PAGE | Cricetinae |
More | recombinant N-terminal extension is a monomer in solution with an elongated shape | Cricetinae |
Synonyms | Comment | Organism |
---|---|---|
L-Lysine-transfer RNA ligase | - |
Cricetinae |
Lysine translase | - |
Cricetinae |
Lysine--tRNA ligase | - |
Cricetinae |
Lysine-tRNA synthetase | - |
Cricetinae |
LysRS | - |
Cricetinae |
Lysyl-transfer ribonucleate synthetase | - |
Cricetinae |
Lysyl-transfer RNA synthetase | - |
Cricetinae |
Lysyl-tRNA synthetase | - |
Cricetinae |
Synthetase, lysyl-transfer ribonucleate | - |
Cricetinae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cricetinae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
ATP | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
5.1 | - |
ATP | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
5.1 | - |
L-lysine | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
5.8 | - |
L-lysine | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
6.2 | - |
human tRNALys3 | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
6.3 | - |
yeast tRNALys | recombinant wild-type enzyme, pH 7.5, 25°C | Cricetinae | |
7.1 | - |
yeast tRNALys | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae | |
7.8 | - |
human tRNALys3 | recombinant N-terminally truncated enzyme, pH 7.5, 25°C | Cricetinae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Cricetinae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Cricetinae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cricetinae | recombinant N-terminal extension, calculation | - |
8.1 |