Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activates 30fold the aminoacylation raection | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression in strain JM101 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + lysine + tRNALys | Escherichia coli | the enzymes major function is to provide Lys-tRNALys fpr protein biosynthesis | AMP + L-lysyl-tRNALys + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
homogenously purified recombinant enzyme, class II enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys | two-step reaction mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
editing and aminoacylation reaction with several amino acids, up to 500fold variations in catalytic efficiencies | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AMP + diphosphate + L-arginyl-tRNALys | - |
Escherichia coli | ATP + L-arginine + tRNALys | - |
r | |
AMP + diphosphate + L-lysyl-tRNALys | - |
Escherichia coli | ATP + L-lysine + tRNALys | - |
r | |
AMP + diphosphate + L-methionyl-tRNALys | - |
Escherichia coli | ATP + L-methionine + tRNALys | - |
r | |
AMP + L-threonyl-tRNALys + diphosphate | - |
Escherichia coli | ATP + L-threonine + tRNALys | - |
r | |
ATP + L-alanine + tRNALys | 264000fold lower activity than with L-lysine, deacylation of the mischarged tRNALys, addition of 2 mM L-lysine abolishes the reaction | Escherichia coli | AMP + L-alanyl-tRNALys + diphosphate | - |
r | |
ATP + L-arginine + tRNALys | best noncognate amino acid substrate, 1600fold lower activity than with L-lysine, addition of 2 mM L-lysine abolishes the reaction | Escherichia coli | AMP + L-arginyl-tRNALys + diphosphate | - |
r | |
ATP + L-cysteine + tRNALys | 750000fold lower activity than with L-lysine, deacylation of the mischarged tRNALys, addition of 2 mM L-lysine abolishes the reaction | Escherichia coli | AMP + L-cysteinyl-tRNALys + diphosphate | - |
r | |
ATP + L-leucine + tRNALys | 132000fold lower activity than with L-lysine, addition of 2 mM L-lysine abolishes the reaction | Escherichia coli | AMP + L-leucyl-tRNALys + diphosphate | - |
r | |
ATP + L-lysine | a small fraction of lysine is converted to lysine lactam in absence or presence of tRNALys | Escherichia coli | ? | - |
? | |
ATP + L-lysine + tRNALys | cognate amino acid, best substrate, two-step reaction mechanism, limited selectivity in the aminoacylation reaction due to inefficient editing of some amino acids, e.g. Met, Leu, Cys, Ala, Thr, by pre-transfer mechanism or the absence of post-transfer editing of other amino acids e.g. Arg, Ser, a small fraction of lysine is converted to lysine lactam | Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
r | |
ATP + L-methionine + tRNALys | 32000fold lower activity than with L-lysine, addition of 2 mM L-lysine abolishes the reaction | Escherichia coli | AMP + L-methionyl-tRNALys + diphosphate | - |
r | |
ATP + L-ornithine | L-ornithine is converted to ornithine lactam in absence or presence of tRNALys | Escherichia coli | ? | - |
? | |
ATP + L-serine + tRNALys | 562000fold lower activity than with L-lysine, deacylation of the mischarged tRNALys, addition of 2 mM L-lysine abolishes the reaction | Escherichia coli | AMP + L-seryl-tRNALys + diphosphate | - |
r | |
ATP + L-threonine + tRNALys | 16000fold lower activity than with L-lysine, addition of 2 mM L-lysine abolishes the reaction | Escherichia coli | AMP + L-threonyl-tRNALys + diphosphate | - |
r | |
ATP + lysine + tRNALys | the enzymes major function is to provide Lys-tRNALys fpr protein biosynthesis | Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
r | |
additional information | the enzyme possesses an efficient pre-transfer editing mechanism which prevents misacylation of tRNALys with ornithine, which results in cyclization to ornithine lactam | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-Lysine-transfer RNA ligase | - |
Escherichia coli |
Lysine translase | - |
Escherichia coli |
Lysine--tRNA ligase | - |
Escherichia coli |
Lysine-tRNA synthetase | - |
Escherichia coli |
LysRS | - |
Escherichia coli |
Lysyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Lysyl-transfer RNA synthetase | - |
Escherichia coli |
Lysyl-tRNA synthetase | - |
Escherichia coli |
Synthetase, lysyl-transfer ribonucleate | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00023 | - |
L-lysyl-tRNALys | deacylation reaction, pH 7.4, 37°C, in absence of DTT | Escherichia coli | |
0.00056 | - |
L-methionyl-tRNALys | deacylation reaction, pH 7.4, 37°C, in absence or presence of 50 mM DTT | Escherichia coli | |
0.00065 | - |
L-lysine | L-lysine lactam formation, pH 7.4, 37°C | Escherichia coli | |
0.00067 | - |
L-arginyl-tRNALys | deacylation reaction, pH 7.4, 37°C, in absence or presence of 50 mM DTT | Escherichia coli | |
0.00076 | - |
L-threonyl-tRNALys | deacylation reaction, pH 7.4, 37°C, in absence or presence of 50 mM DTT | Escherichia coli | |
0.0088 | - |
L-lysyl-tRNALys | deacylation reaction, pH 7.4, 37°C, in presence of 50 mM DTT | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |