Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | synthesis of lysyl-tRNA from lysyl-AMP-enzyme inhibited, formation of Lys-tRNALys from lysine, tRNA and ATP-enzyme complex or ATP is dependent on Mg2+ | Escherichia coli | |
NH4+ | formation of Lys-tRNALys from lysine, tRNA and ATP-enzyme complex or ATP | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
Lys | aminoacylation | Escherichia coli | |
0.005 | - |
L-Lys | aminoacylation, enzyme form EII | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | formation of Lys-tRNALys from lysine, tRNA and ATP-enzyme complex or ATP is dependent on Mg2+ | Escherichia coli | |
NH4+ | synthesis of lysyl-tRNA from lysyl-AMP-enzyme is dependent on NH4+ or other monovalent cations | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
1 * 72000 + 1 + 62000, SDS-PAGE | Escherichia coli |
150000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
B | - |
Escherichia coli B / ATCC 11303 | - |
B | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.6 | - |
ATP-diphosphate exchange | Escherichia coli |
Storage Stability | Organism |
---|---|
-15°C, 2 years | Escherichia coli |
4°C, 8 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + lysine + tRNALys | - |
Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + lysine + tRNALys | - |
Escherichia coli B / ATCC 11303 | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
additional information | lysine-dependent ATP-diphosphate exchange reaction: lysine + ATP + enzyme/lysine-AMP-enzyme + diphosphate | Escherichia coli | ? | - |
? | |
additional information | lysine-dependent ATP-diphosphate exchange reaction: lysine + ATP + enzyme/lysine-AMP-enzyme + diphosphate | Escherichia coli B / ATCC 11303 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 72000 + 1 + 62000, SDS-PAGE | Escherichia coli |