Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ile-ol-AMP | - |
Staphylococcus aureus | |
isoleucinyl adenylate | i.e. Ile-ol-AMP, nonhydrolyzable reaction intermediate analogue, competitive with respect to both ATP and Ile | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reaction kinetics | Staphylococcus aureus | |
0.0001 | - |
tRNAIle | below, tRNAIle aminoacylation reaction, pH 7.9, 22°C | Staphylococcus aureus | |
0.005 | - |
L-isoleucine | tRNAIle aminoacylation reaction, pH 7.9, 22°C | Staphylococcus aureus | |
0.01 | - |
L-isoleucine | ATP-diphosphate exchange reaction, pH 7.9, 22°C | Staphylococcus aureus | |
0.24 | - |
ATP | tRNAIle aminoacylation reaction, pH 7.9, 22°C | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | Staphylococcus aureus | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-isoleucine + tRNAIle = AMP + diphosphate + L-isoleucyl-tRNAIle | reaction kinetics and mechanism | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | - |
Staphylococcus aureus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
r | |
ATP + L-isoleucine + tRNAIle | formation of a enzyme-bound aminoacyl adenylate intermediate | Staphylococcus aureus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
r | |
additional information | enzyme also performs the reversible ATP-diphosphate exchange reaction | Staphylococcus aureus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IleRS | - |
Staphylococcus aureus |
IRS | - |
Staphylococcus aureus |
Isoleucine translase | - |
Staphylococcus aureus |
Isoleucine--tRNA ligase | - |
Staphylococcus aureus |
Isoleucine-transfer RNA ligase | - |
Staphylococcus aureus |
Isoleucine-tRNA synthetase | - |
Staphylococcus aureus |
Isoleucyl-transfer ribonucleate synthetase | - |
Staphylococcus aureus |
Isoleucyl-transfer RNA synthetase | - |
Staphylococcus aureus |
Isoleucyl-tRNA synthetase | - |
Staphylococcus aureus |
Mupirocin resistance protein | - |
Staphylococcus aureus |
Synthetase, isoleucyl-transfer ribonucleate | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
L-isoleucine | tRNAIle aminoacylation reaction, pH 7.9, 22°C | Staphylococcus aureus | |
18 | - |
L-isoleucine | ATP-diphosphate exchange reaction, pH 7.9, 22°C | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binds more tightly to the enzyme-Ile complex than to the free enzyme | Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00003 | - |
Ile-ol-AMP | pH 7.9, 22°C | Staphylococcus aureus |