Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Geobacillus stearothermophilus | |
Mg2+ | at a concentration of ATP and diphosphate of 3 mM, the optimal Mg2+ concentration is 6-10 mM | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
disc gel electrophoresis | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
254.5 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
-20°C | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | 0.3% or less of the activity with isoleucine is measured with other amino acids | Geobacillus stearothermophilus | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
additional information | Ile + ATP + enzyme/Ile-AMP-enzyme + diphosphate, isoleucine-dependent ATP-diphosphate exchange | Geobacillus stearothermophilus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
formation of isoleucyl-tRNA | Geobacillus stearothermophilus |
65 | - |
isoleucine-dependent ATP-diphosphate exchange reaction | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
isoleucine, isoleucine + ATP and tRNA + Mg2+ protect against heat inactivation, some protection by high concentrations of valine | Geobacillus stearothermophilus |
60 | - |
stable below | Geobacillus stearothermophilus |