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Literature summary for 6.1.1.5 extracted from

  • Charlier, J.; Grosjean, H.
    Isoleucyl-transfer ribonucleic acid synthetase from Bacillus stearothermophilus. Properties of the enzyme (1972), Eur. J. Biochem., 25, 163-174.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Geobacillus stearothermophilus
Mg2+ at a concentration of ATP and diphosphate of 3 mM, the optimal Mg2+ concentration is 6-10 mM Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
disc gel electrophoresis Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
254.5
-
-
Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
-20°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-isoleucine + tRNAIle 0.3% or less of the activity with isoleucine is measured with other amino acids Geobacillus stearothermophilus AMP + diphosphate + L-isoleucyl-tRNAIle
-
?
additional information Ile + ATP + enzyme/Ile-AMP-enzyme + diphosphate, isoleucine-dependent ATP-diphosphate exchange Geobacillus stearothermophilus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
formation of isoleucyl-tRNA Geobacillus stearothermophilus
65
-
isoleucine-dependent ATP-diphosphate exchange reaction Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
isoleucine, isoleucine + ATP and tRNA + Mg2+ protect against heat inactivation, some protection by high concentrations of valine Geobacillus stearothermophilus
60
-
stable below Geobacillus stearothermophilus