Protein Variants | Comment | Organism |
---|---|---|
AIleRS | mutant enzymes IleRS(C922S) and AIleRS with replacement of Cys922 through Ala939 with a 33 amino acid peptide unable to bind zinc. Mutant enzymes have altered zinc binding and aminoacylation activity | Escherichia coli |
IleRS(C922S) | mutant enzymes IleRS(C922S) and AIleRS with replacement of Cys922 through Ala939 with a 33 amino acid peptide unable to bind zinc. Mutant enzymes have altered zinc binding and aminoacylation activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
tRNAIle | wild-type enzyme | Escherichia coli | |
0.7 | - |
ATP | wild-type enzyme | Escherichia coli | |
1.3 | - |
Ile | wild-type enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | 2 enzyme bound zinc atoms per polypeptide chain | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant enzymes IleRS(C922S) and AIleRS, with replacement of Cys922 through Ala939 with a 33 amino acid peptide unable to bind zinc (AIleRS), mutant enzymes have altered zinc binding and aminoacylation activity | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNAIle | - |
Escherichia coli | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
additional information | Ile + ATP + enzyme/Ile-AMP-enzyme + diphosphate, isoleucine-dependent ATP-diphosphate exchange | Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
tRNAIle | wild-type enzyme | Escherichia coli | |
104 | - |
isoleucine | wild-type enzyme | Escherichia coli |