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Literature summary for 6.1.1.4 extracted from

  • Fang, Z.P.; Wang, M.; Ruan, Z.R.; Tan, M.; Liu, R.J.; Zhou, M.; Zhou, X.L.; Wang, E.D.
    Coexistence of bacterial leucyl-tRNA synthetases with archaeal tRNA binding domains that distinguish tRNALeu in the archaeal mode (2014), Nucleic Acids Res., 42, 5109-5124.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Natrialba magadii

Inhibitors

Inhibitors Comment Organism Structure
NaCl no activity of isoform LeuRS1 is detected in NaCl solutions Natrialba magadii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00335
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.00773
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.00843
-
 L-leucine isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.01101
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.01251
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.01313
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.01643
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.891
-
 L-leucine isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Natrialba magadii 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ the enzyme activity requires high KCl concentration with highest activity in the presence of 3.75 M KCl Natrialba magadii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
102200
-
 x * 102200, isoform LeuRS1, calculated from amino acid sequence Natrialba magadii
107600
-
 x * 107600, isoform LeuRS2, calculated from amino acid sequence Natrialba magadii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Natrialba magadii
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu Natrialba magadii ATCC 43099
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

Organism UniProt Comment Textmining
Natrialba magadii
-
-
-
Natrialba magadii ATCC 43099
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography, and gel filtration Natrialba magadii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + diphosphate + L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
 Natrialba magadii ATP + L-leucine + Pyrococcus horikoshii tRNALeu(GAG)
-
 r
AMP + diphosphate + L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
 Natrialba magadii ATCC 43099 ATP + L-leucine + Pyrococcus horikoshii tRNALeu(GAG)
-
 r
ATP + L-leucine + Natrialba magadii tRNALeu(CAA)
-
 Natrialba magadii AMP + diphosphate + L-leucyl-Natrialba magadii tRNALeu(CAA)
-
 ?
ATP + L-leucine + Natrialba magadii tRNALeu(CAA)
-
 Natrialba magadii ATCC 43099 AMP + diphosphate + L-leucyl-Natrialba magadii tRNALeu(CAA)
-
 ?
ATP + L-leucine + Natrialba magadii tRNALeu(GAG) highest activity Natrialba magadii AMP + diphosphate + L-leucyl-Natrialba magadii tRNALeu(GAG)
-
 ?
ATP + L-leucine + Natrialba magadii tRNALeu(GAG) highest activity Natrialba magadii ATCC 43099 AMP + diphosphate + L-leucyl-Natrialba magadii tRNALeu(GAG)
-
 ?
ATP + L-leucine + Pyrococcus horikoshii tRNALeu(GAG) 100% activity Natrialba magadii AMP + diphosphate + L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
 r
ATP + L-leucine + Pyrococcus horikoshii tRNALeu(GAG) 100% activity Natrialba magadii ATCC 43099 AMP + diphosphate + L-leucyl-Pyrococcus horikoshii tRNALeu(GAG)
-
 r
ATP + L-leucine + tRNALeu
-
 Natrialba magadii AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu
-
 Natrialba magadii ATCC 43099 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

Subunits Comment Organism
? x * 102200, isoform LeuRS1, calculated from amino acid sequence Natrialba magadii
? x * 107600, isoform LeuRS2, calculated from amino acid sequence Natrialba magadii

Synonyms

Synonyms Comment Organism
Leucyl-tRNA synthetase
-
 Natrialba magadii
LeuRS1 isoform, exhibits much higher aminoacylation and editing activity than isoform LeuRS2 Natrialba magadii
LeuRS2 isoform, exhibits much less aminoacylation and editing activity than isoform LeuRS1 Natrialba magadii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000418
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.000668
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.00119
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.00961
-
 L-leucine isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.0418
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.0464
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.064
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.563
-
 L-leucine isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 in Tris-HCl or Bis-Tris-propane-HCl, the activity decreases obviously at pH 8.0 or below Natrialba magadii
9.5
-
 in CHES-KOH, the activity decreases obviously at pH 8.0 or below Natrialba magadii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Natrialba magadii

General Information

General Information Comment Organism
metabolism isoform LeuRS1 generates Leu-tRNALeu for protein biosynthesis and exhibits obvious post-transfer editing activity to prevent generation of mischarged tRNALeu Natrialba magadii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0108
-
 L-leucine isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.0318
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.0724
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
0.199
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS2, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
3.34
-
 L-leucyl-Pyrococcus horikoshii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
4.21
-
 Natrialba magadii tRNALeu(CAA) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
9.28
-
 Natrialba magadii tRNALeu(GAG) isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii
66.8
-
 L-leucine isoform LeuRS1, in 20 mM Tris-HCl, pH 9.0, 3.5 M KCl, 30 mM MgCl2, 1 mM dithiohtreitol, at 40°C Natrialba magadii