BRENDA - Enzyme Database
show all sequences of 6.1.1.4

Interdomain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA synthetase

Tan, M.; Zhu, B.; Liu, R.J.; Chen, X.; Zhou, X.L.; Wang, E.D.; Biochem. J. 449, 123-131 (2013) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Engineering
Protein Variants
Commentary
Organism
E184A
the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme
Escherichia coli
E184R
the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme. The substitution specifically inhibits tRNA-dependent pre-transfer editing
Escherichia coli
E184R/T252R
the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme
Escherichia coli
R185E
the mutation significantly enhances tRNA-dependent pre-transfer editing activity
Escherichia coli
R286E
the mutation significantly enhances tRNA-dependent pre-transfer editing activity
Escherichia coli
T252R
the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
Ile-tRNALeu
mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
0.0021
-
Ile-tRNALeu
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
0.0024
-
Ile-tRNALeu
mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
0.0025
-
Ile-tRNALeu
mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
0.039
-
L-leucine
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + L-leucine + tRNALeu
Escherichia coli
-
AMP + diphosphate + L-leucyl-tRNALeu
-
-
?
additional information
Escherichia coli
the enzyme has evolved both tRNA-dependent pre- and post-transfer editing capabilities to ensure catalytic specificity
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
P07813
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
AMP + diphosphate + Ile-tRNALeu
-
726909
Escherichia coli
ATP + L-isoleucine + tRNALeu
-
-
-
?
ATP + L-leucine + tRNALeu
-
726909
Escherichia coli
AMP + diphosphate + L-leucyl-tRNALeu
-
-
-
?
additional information
the enzyme has evolved both tRNA-dependent pre- and post-transfer editing capabilities to ensure catalytic specificity
726909
Escherichia coli
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
Leucyl-tRNA synthetase
-
Escherichia coli
LeuRS
-
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
7
-
Ile-tRNALeu
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
7.4
-
Ile-tRNALeu
mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
7.6
-
Ile-tRNALeu
mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
8.8
-
Ile-tRNALeu
mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
73.7
-
L-leucine
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Escherichia coli
Engineering (protein specific)
Protein Variants
Commentary
Organism
E184A
the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme
Escherichia coli
E184R
the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme. The substitution specifically inhibits tRNA-dependent pre-transfer editing
Escherichia coli
E184R/T252R
the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme
Escherichia coli
R185E
the mutation significantly enhances tRNA-dependent pre-transfer editing activity
Escherichia coli
R286E
the mutation significantly enhances tRNA-dependent pre-transfer editing activity
Escherichia coli
T252R
the mutant performs the activities of amino acid activation, aminoacylation and deacylation of mischarged tRNAs as well as the native enzyme
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
Ile-tRNALeu
mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
0.0021
-
Ile-tRNALeu
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
0.0024
-
Ile-tRNALeu
mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
0.0025
-
Ile-tRNALeu
mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
0.039
-
L-leucine
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + L-leucine + tRNALeu
Escherichia coli
-
AMP + diphosphate + L-leucyl-tRNALeu
-
-
?
additional information
Escherichia coli
the enzyme has evolved both tRNA-dependent pre- and post-transfer editing capabilities to ensure catalytic specificity
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
AMP + diphosphate + Ile-tRNALeu
-
726909
Escherichia coli
ATP + L-isoleucine + tRNALeu
-
-
-
?
ATP + L-leucine + tRNALeu
-
726909
Escherichia coli
AMP + diphosphate + L-leucyl-tRNALeu
-
-
-
?
additional information
the enzyme has evolved both tRNA-dependent pre- and post-transfer editing capabilities to ensure catalytic specificity
726909
Escherichia coli
?
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
7
-
Ile-tRNALeu
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
7.4
-
Ile-tRNALeu
mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
7.6
-
Ile-tRNALeu
mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
8.8
-
Ile-tRNALeu
mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
73.7
-
L-leucine
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1890
-
L-leucine
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
3100
-
Ile-tRNALeu
mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
3300
-
Ile-tRNALeu
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
3500
-
Ile-tRNALeu
mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
3800
-
Ile-tRNALeu
mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1890
-
L-leucine
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
3100
-
Ile-tRNALeu
mutant enzyme R286E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
3300
-
Ile-tRNALeu
wild type enzyme, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
3500
-
Ile-tRNALeu
mutant enzyme E184R, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
3800
-
Ile-tRNALeu
mutant enzyme R185E, in 100 mM HEPES (pH 7.8), 10 mM MgCl2, at 37°C
Escherichia coli
Other publictions for EC 6.1.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744849
Sato
Leucyl-tRNA synthetase is req ...
Mus musculus
Exp. Cell Res.
364
184-190
2018
-
-
1
-
1
-
-
-
1
1
-
1
-
2
-
-
-
-
-
4
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
745556
Dulic
Kinetic origin of substrate s ...
Escherichia coli
J. Mol. Biol.
430
1-16
2018
-
-
1
1
6
-
-
1
-
1
-
1
-
4
-
-
1
-
-
-
-
-
3
-
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
6
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
744247
Choi
Leucine-induced localization ...
Homo sapiens
Biochem. Biophys. Res. Commun.
493
1129-1135
2017
-
-
-
-
-
-
1
-
3
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
744654
Yoon
Leucyl-tRNA synthetase activa ...
Homo sapiens
Cell Rep.
16
1510-1517
2016
-
-
-
-
2
-
-
-
1
1
-
1
-
1
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
744693
Kim
beta-Turn mimetic-based stabi ...
Homo sapiens
Chem. Sci.
7
2753-2761
2016
-
-
-
-
-
-
9
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
745458
Gudzera
Identification of Mycobacteri ...
Mycobacterium tuberculosis, Homo sapiens, Mycobacterium tuberculosis ATCC 25618 / H37Rv
J. Enzyme Inhib. Med. Chem.
31
201-207
2016
-
1
2
-
-
-
57
-
1
1
-
1
-
11
-
-
2
-
-
-
-
-
1
-
6
2
-
-
-
2
-
-
1
-
-
-
-
1
2
1
-
-
-
-
57
-
-
1
1
-
1
-
-
-
2
-
-
-
-
1
-
2
-
-
-
2
-
-
-
-
1
1
-
-
-
744968
Giordano
The phenotypic expression of ...
Homo sapiens
Front. Genet.
6
113
2015
-
-
-
-
-
-
-
-
1
1
-
1
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
745321
Yan
Modulation of aminoacylation ...
Pyrococcus horikoshii, Aquifex aeolicus, Escherichia coli, Mycoplasma mobile, Homo sapiens, Mycoplasma mobile ATCC 43663 / 163K / NCTC 11711, Pyrococcus horikoshii ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3
J. Biol. Chem.
290
12256-12267
2015
-
-
5
-
24
-
-
31
1
5
-
7
-
13
-
-
5
-
-
-
-
-
17
1
17
5
-
-
29
5
-
-
5
-
-
-
-
-
5
5
-
24
-
-
-
-
31
1
5
-
7
-
-
-
5
-
-
-
-
17
1
5
-
-
29
5
-
-
-
-
13
13
-
29
29
745503
Kumar
Zinc is the molecular switch ...
Escherichia coli
J. Inorg. Biochem.
142
59-67
2015
-
-
1
-
5
-
-
1
-
2
-
1
-
3
-
-
1
-
-
-
-
-
1
1
5
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
727364
Perli
The isolated carboxy-terminal ...
Homo sapiens
EMBO Mol. Med.
6
169-182
2014
-
-
-
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
728409
Fang
Coexistence of bacterial leucy ...
Natrialba magadii, Natrialba magadii ATCC 43099
Nucleic Acids Res.
42
5109-5124
2014
-
-
1
-
-
-
1
8
1
1
2
2
-
6
-
-
1
-
-
-
-
-
10
2
3
-
-
-
8
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
8
1
1
2
2
-
-
-
1
-
-
-
-
10
2
-
-
-
8
2
-
-
-
-
1
1
-
8
8
744788
Perli
The isolated carboxy-terminal ...
Homo sapiens
EMBO Mol. Med.
6
169-182
2014
-
-
1
-
1
-
-
-
3
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
745112
Wang
Leucyl-tRNA synthetase regula ...
Bos taurus
Int. J. Mol. Sci.
15
5952-5969
2014
-
-
1
-
-
-
-
-
2
1
-
1
-
6
-
-
-
-
-
5
-
-
1
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
746422
Huang
A bridge between the aminoacy ...
Homo sapiens
RNA
20
1440-1450
2014
-
-
-
-
25
-
-
1
1
1
-
1
-
7
-
-
-
-
-
-
-
-
1
1
5
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
25
-
-
-
-
1
1
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
726909
Tan
Interdomain communication modu ...
Escherichia coli
Biochem. J.
449
123-131
2013
-
-
1
-
6
-
-
5
-
-
-
2
-
1
-
-
1
-
-
-
-
-
3
-
2
-
-
-
5
-
-
-
1
-
-
-
-
-
1
1
-
6
-
-
-
-
5
-
-
-
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Functional characterization of ...
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Aminoacyl-tRNA synthetases are ...
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Two tyrosine residues outside ...
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A unique insertion in the CP1 ...
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702347
Pang
A paradigm shift for the amino ...
Homo sapiens
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3
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Staneviciene
Subacute effects of cadmium an ...
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-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694435
Yao
Recognition of tRNALeu by Aqui ...
Aquifex aeolicus
Nucleic Acids Res.
36
2728-2738
2008
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
-
-
-
-
-
-
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1
-
2
1
-
-
1
1
-
-
-
-
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-
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-
-
-
-
1
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-
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-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
695107
Park
Overexpressed mitochondrial le ...
Homo sapiens
RNA
14
2407-2416
2008
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
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1
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1
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672235
Zhai
Modulation of substrate specif ...
Escherichia coli
Biochemistry
46
3331-3337
2007
-
-
1
-
14
-
-
3
-
1
-
1
-
4
-
-
1
-
-
-
-
-
2
1
2
-
-
-
6
1
-
-
1
-
-
-
-
-
1
1
-
14
-
-
-
-
3
-
1
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
6
1
-
-
-
-
-
-
-
-
-
672244
Lue
A single residue in leucyl-tRN ...
Escherichia coli, Homo sapiens
Biochemistry
46
4466-4472
2007
-
-
-
-
6
-
-
14
2
2
-
2
-
8
-
-
-
-
-
-
2
-
8
-
4
2
-
-
15
2
-
-
2
-
-
-
-
-
-
2
-
6
-
-
-
-
14
2
2
-
2
-
-
-
-
-
-
2
-
8
-
2
-
-
15
2
-
-
-
-
-
-
-
-
-
672250
Fukunaga
The C-terminal domain of the a ...
Pyrococcus horikoshii
Biochemistry
46
4985-4996
2007
-
-
-
-
1
-
-
2
-
1
-
1
-
4
-
-
-
-
-
-
1
-
2
1
2
2
1
-
2
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
2
-
1
-
1
-
-
-
-
-
-
1
-
2
1
2
1
-
2
1
-
-
-
-
-
-
-
-
-
672252
Vu
A unique insert of leucyl-tRNA ...
Escherichia coli
Biochemistry
46
5170-5176
2007
1
-
1
-
1
-
-
3
-
1
-
1
-
3
-
-
1
-
-
-
1
-
2
-
2
1
-
-
2
1
-
-
1
-
-
-
1
-
1
1
-
1
-
-
-
-
3
-
1
-
1
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1
-
-
1
-
2
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
672264
Betha
Isolated CP1 domain of Escheri ...
Escherichia coli
Biochemistry
46
6258-6267
2007
-
-
1
-
11
-
-
-
-
1
-
1
-
6
-
-
1
-
-
-
1
-
3
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
11
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
674875
Praetorius-Ibba
Functional association between ...
Methanothermobacter thermautotrophicus
J. Biol. Chem.
282
3680-3687
2007
1
-
1
-
-
-
-
1
-
1
-
1
-
11
-
-
1
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
1
-
-
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1
-
1
1
-
-
-
-
-
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1
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1
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1
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1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
675436
Nawaz
Molecular and functional disse ...
Saccharomyces cerevisiae
J. Mol. Biol.
367
384-394
2007
-
-
-
-
11
-
-
5
3
1
-
1
-
6
-
-
-
-
-
-
1
-
3
1
2
-
-
-
4
-
-
-
1
-
-
-
-
-
-
1
-
11
-
-
-
-
5
3
1
-
1
-
-
-
-
-
-
1
-
3
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
677102
Zhu
A present-day aminoacyl-tRNA s ...
Aquifex aeolicus
RNA
13
15-21
2007
-
-
-
-
1
-
-
1
-
1
-
1
-
7
-
-
-
-
-
-
1
-
2
-
2
2
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
1
-
2
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
661018
Liu
Crystal structures of the edit ...
Escherichia coli
Biochem. J.
394
399-407
2006
-
-
1
1
-
-
-
-
-
-
-
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4
-
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1
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
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1
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1
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-
-
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1
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-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
663318
Olieric
Expression, purification, and ...
Aquifex aeolicus
Protein Expr. Purif.
47
1-9
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
13
-
-
1
-
-
-
-
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2
-
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-
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-
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-
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-
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1
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-
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1
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1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672106
Ma
Split leucine-specific domain ...
Aquifex aeolicus
Biochemistry
45
14809-14816
2006
-
-
1
-
8
-
-
4
-
1
-
1
-
12
-
-
1
-
-
-
-
-
3
1
2
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
-
8
-
-
-
-
4
-
1
-
1
-
-
-
1
-
-
-
-
3
1
1
-
-
3
1
-
-
-
-
-
-
-
-
-
672507
Viezeliene
-
Effect of aluminum ions on the ...
Mus musculus
Biologija (Vilnius)
4
28-30
2006
1
-
-
-
-
-
1
-
-
1
-
1
-
1
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
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1
-
-
-
1
-
-
1
-
-
-
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1
-
-
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1
-
1
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-
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-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674619
Hsu
Functional divergence of a uni ...
Saccharomyces cerevisiae, Escherichia coli
J. Biol. Chem.
281
23075-23082
2006
2
-
2
-
18
-
-
1
2
2
-
2
-
8
-
-
2
-
-
-
-
-
4
2
4
2
-
-
-
2
-
-
2
-
-
-
2
-
2
2
-
18
-
-
-
-
1
2
2
-
2
-
-
-
2
-
-
-
-
4
2
2
-
-
-
2
-
-
-
-
-
-
-
-
-
674686
Karkhanis
A viable amino acid editing ac ...
Saccharomyces cerevisiae
J. Biol. Chem.
281
33217-33225
2006
-
-
1
-
3
-
-
-
3
1
-
1
-
8
-
-
1
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
3
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
661178
Zhai
Two conserved threonines colla ...
Escherichia coli
Biochemistry
44
15437-15443
2005
-
-
-
-
5
-
-
6
-
-
-
-
-
6
-
-
1
-
-
-
-
-
2
-
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
661190
Lue
An aminoacyl-tRNA synthetase w ...
Escherichia coli, Homo sapiens
Biochemistry
44
3010-3016
2005
-
-
-
-
-
-
-
4
1
-
-
-
-
6
-
-
-
-
-
-
-
-
4
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
661591
Zhao
Leucyl-tRNA synthetase from th ...
Aquifex aeolicus
EMBO J.
24
1430-1439
2005
-
-
1
-
-
-
-
1
-
-
-
-
-
14
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
662416
Ling
The C-terminal appended domain ...
Homo sapiens
J. Biol. Chem.
280
34755-34763
2005
-
-
1
-
-
-
-
10
5
-
1
1
-
8
-
-
1
-
-
-
-
-
2
1
1
-
-
-
10
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
10
5
-
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
10
-
-
-
-
-
-
-
-
-
-
662641
Fukunaga
Crystal structure of leucyl-tR ...
Pyrococcus horikoshii
J. Mol. Biol.
346
57-71
2005
-
-
-
1
1
-
-
-
-
-
-
-
-
14
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662939
Tukalo
The crystal structure of leucy ...
Thermus thermophilus
Nat. Struct. Mol. Biol.
12
923-930
2005
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660871
Xu
Groups on the side chain of T2 ...
Escherichia coli
Biochem. Biophys. Res. Commun.
318
11-16
2004
-
-
-
-
3
-
-
9
-
-
-
-
-
6
-
-
1
-
-
-
-
-
2
-
2
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
9
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
661109
Mursinna
Molecular dissection of a crit ...
Escherichia coli
Biochemistry
43
155-165
2004
-
-
-
-
3
-
-
8
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
662245
Xu
Leucyl-tRNA synthetase from th ...
Aquifex aeolicus
J. Biol. Chem.
279
32151-32158
2004
-
-
-
-
-
-
-
3
-
-
-
-
-
15
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
662619
Sohm
Recognition of human mitochond ...
Homo sapiens
J. Mol. Biol.
339
17-29
2004
-
-
-
-
-
-
-
2
3
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
662970
Zheng
Two distinct domains of the be ...
Aquifex aeolicus
Nucleic Acids Res.
32
3294-3303
2004
-
-
1
-
12
-
-
13
-
-
-
-
-
8
-
-
1
-
-
-
-
-
1
1
1
-
-
-
13
-
-
-
-
-
-
-
-
-
1
-
-
12
-
-
-
-
13
-
-
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
13
-
-
-
-
-
-
-
-
-
-
663301
Ling
High-level expression and sing ...
Aquifex aeolicus
Protein Expr. Purif.
36
146-149
2004
-
-
1
-
-
-
-
6
-
-
-
-
-
12
-
-
1
-
-
-
1
-
1
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
663351
Lee
Molecular modeling study of th ...
Escherichia coli
Proteins
54
693-704
2004
-
-
-
-
-
-
-
-
-
-
1
-
-
9
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721148
Fukunaga
Crystallization and preliminar ...
Pyrococcus horikoshii
Acta Crystallogr. Sect. D
60
1916-1918
2004
-
-
1
1
-
-
-
-
-
-
1
-
-
14
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
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1
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Enzymes assembled from Aquifex ...
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1
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1
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3
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1
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4
1
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1
1
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1
2
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6
1
5
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1
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1
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1
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Colas
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1
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2
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1
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1
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1
1
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1
1
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4
7
1
2
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1
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1
1
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1
1
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1
1
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Properties of purified chlorop ...
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4
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1
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Naldixic acid, oxolinic acid, ...
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3
2
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1
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3
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1
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432
Imbault
Chloroplast leucyl-tRNA synthe ...
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1981
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3
6
8
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8
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1
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4
1
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1
1
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1
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3
6
8
2
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1
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4
1
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1
1
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1
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433
Sarantoglou
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Purification of Euglena gracil ...
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1
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1
1
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3
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1
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1
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1
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1
1
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1
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1
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13
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14
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7
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13
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2
1
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1
1
1
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1
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2
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3
3
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1
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1
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2
1
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1
1
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1
1
1
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2
2
1
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2
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1
2
2
2
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1
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1
1
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531
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