Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.4 extracted from

  • Zhou, X.L.; Wang, M.; Tan, M.; Huang, Q.; Eriani, G.; Wang, E.D.
    Functional characterization of leucine-specific domain 1 from eukaryal and archaeal leucyl-tRNA synthetases (2010), Biochem. J., 429, 505-513.
    View publication on PubMed
Search for more literature for 6.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of His-tagged GlLeuRS in Escherichia coli strain BL21-Codon Plus(DE3)-RIL. Complementation assay of the thermosensitive Escherichia coli KL231 strain Giardia intestinalis
overexpression of His-tagged PhLeuRS in Escherichia coli strain BL21-Codon Plus(DE3)-RIL. Complementation assay of the thermosensitive Escherichia coli KL231 strain Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
D121A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
D173A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
D332A site-directed mutagenesis Pyrococcus horikoshii
D444A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
D98A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
E113A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
E114A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
E165A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
E167A site-directed mutagenesis, the mutant shows defects in leucine activation, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
F119A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
F171A site-directed mutagenesis, inactive mutant Giardia intestinalis
I104A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
I115A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
K100A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
K100A/Y105A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
K100A/Y109A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
K139A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
K141A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
K142A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
K144A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
K148A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
K152A site-directed mutagenesis, the mutant shows defects in leucine activation, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
K166A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
K170A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
additional information deletion of the entire LSD1 abolishes synthetic activity of LeuRS Giardia intestinalis
additional information deletion of the entire LSD1 abolishes synthetic activity of LeuRS Pyrococcus horikoshii
N96A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
Q154A site-directed mutagenesis, the mutant shows defects in leucine activation, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
R106A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
R97A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
S153A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
T101A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
T118A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
V108A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
W103A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
W155A site-directed mutagenesis, mutant kinetics compared to the wild-type enzyme, overview Giardia intestinalis
Y105A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii
Y109A site-directed mutagenesis, mutant steady-state leucine activation kinetics compared to the wild-type enzyme, overview Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state leucine activation and aminoacylation kinetics of GlLeuRS and its mutants, overview Giardia intestinalis
0.0024
-
 L-leucine pH 8.2, 45°C, mutant K152A Giardia intestinalis
0.0035
-
 tRNALeu pH 8.2, 45°C, mutant K142A Giardia intestinalis
0.0035
-
 L-leucine pH 8.2, 45°C, mutant Q154A Giardia intestinalis
0.0038
-
 tRNALeu pH 8.2, 45°C, mutant Q154A Giardia intestinalis
0.004
-
 tRNALeu pH 8.2, 45°C, mutant S153A Giardia intestinalis
0.0049
-
 tRNALeu pH 8.2, 45°C, mutant E167A Giardia intestinalis
0.005
-
 tRNALeu pH 8.2, 45°C, mutant K148A Giardia intestinalis
0.0051
-
 tRNALeu pH 8.2, 45°C, mutant K141A Giardia intestinalis
0.0051
-
 tRNALeu pH 8.2, 45°C, mutant K152A Giardia intestinalis
0.0051
-
 tRNALeu pH 8.2, 45°C, mutant K170A Giardia intestinalis
0.0052
-
 tRNALeu pH 8.2, 45°C, mutant K144A Giardia intestinalis
0.00537
-
 L-leucine pH 7.5, 65°C, mutant N96A Pyrococcus horikoshii
0.00555
-
 L-leucine pH 7.5, 65°C, mutant Y105A Pyrococcus horikoshii
0.00559
-
 L-leucine pH 7.5, 65°C, mutant I115A Pyrococcus horikoshii
0.00569
-
 L-leucine pH 7.5, 65°C, mutant I104A Pyrococcus horikoshii
0.0057
-
 L-leucine pH 7.5, 65°C, mutant K100A/Y109A Pyrococcus horikoshii
0.00581
-
 L-leucine pH 7.5, 65°C, mutant V108A Pyrococcus horikoshii
0.0059
-
 tRNALeu pH 8.2, 45°C, mutant D173A Giardia intestinalis
0.00592
-
 L-leucine pH 7.5, 65°C, wild-type enzyme Pyrococcus horikoshii
0.00592
-
 L-leucine pH 7.5, 65°C, mutant D98A Pyrococcus horikoshii
0.00618
-
 L-leucine pH 7.5, 65°C, mutant E113A Pyrococcus horikoshii
0.00623
-
 L-leucine pH 7.5, 65°C, mutant E114A Pyrococcus horikoshii
0.0063
-
 L-leucine pH 7.5, 65°C, mutant K100A/Y105A Pyrococcus horikoshii
0.00636
-
 L-leucine pH 7.5, 65°C, mutant T118A Pyrococcus horikoshii
0.0071
-
 tRNALeu pH 8.2, 45°C, mutant W155A Giardia intestinalis
0.00721
-
 L-leucine pH 7.5, 65°C, mutant D121A Pyrococcus horikoshii
0.0081
-
 tRNALeu pH 8.2, 45°C, mutant K166A Giardia intestinalis
0.0083
-
 tRNALeu pH 8.2, 45°C, mutant E165A Giardia intestinalis
0.00854
-
 L-leucine pH 7.5, 65°C, mutant R106A Pyrococcus horikoshii
0.00871
-
 L-leucine pH 7.5, 65°C, mutant W103A Pyrococcus horikoshii
0.00891
-
 L-leucine pH 7.5, 65°C, mutant K100A Pyrococcus horikoshii
0.00902
-
 L-leucine pH 7.5, 65°C, mutant Y109A Pyrococcus horikoshii
0.00927
-
 L-leucine pH 7.5, 65°C, mutant R97A Pyrococcus horikoshii
0.00984
-
 L-leucine pH 7.5, 65°C, mutant T101A Pyrococcus horikoshii
0.0114
-
 tRNALeu pH 8.2, 45°C, mutant K139A Giardia intestinalis
0.0118
-
 L-leucine pH 7.5, 65°C, mutant F119A Pyrococcus horikoshii
0.0119
-
 L-leucine pH 8.2, 45°C, mutant W155A Giardia intestinalis
0.0124
-
 L-leucine pH 8.2, 45°C, mutant K148A Giardia intestinalis
0.0132
-
 L-leucine pH 8.2, 45°C, mutant K141A Giardia intestinalis
0.0141
-
 L-leucine pH 8.2, 45°C, mutant K170A Giardia intestinalis
0.0145
-
 L-leucine pH 8.2, 45°C, mutant K142A Giardia intestinalis
0.0147
-
 L-leucine pH 8.2, 45°C, mutant K166A Giardia intestinalis
0.0151
-
 L-leucine pH 8.2, 45°C, mutant K139A Giardia intestinalis
0.0153
-
 L-leucine pH 8.2, 45°C, mutant K144A Giardia intestinalis
0.0157
-
 L-leucine pH 8.2, 45°C, mutant E165A Giardia intestinalis
0.0159
-
 L-leucine pH 8.2, 45°C, mutant S153A Giardia intestinalis
0.0162
-
 L-leucine pH 8.2, 45°C, mutant E167A Giardia intestinalis
0.0165
-
 L-leucine pH 8.2, 45°C, mutant D173A Giardia intestinalis
0.296
-
 ATP pH 7.5, 65°C, mutant R106A Pyrococcus horikoshii
0.323
-
 ATP pH 7.5, 65°C, mutant R97A Pyrococcus horikoshii
0.351
-
 ATP pH 7.5, 65°C, mutant V108A Pyrococcus horikoshii
0.362
-
 ATP pH 7.5, 65°C, mutant D98A Pyrococcus horikoshii
0.366
-
 ATP pH 7.5, 65°C, mutant K100A/Y109A Pyrococcus horikoshii
0.37
-
 ATP pH 7.5, 65°C, mutant N96A Pyrococcus horikoshii
0.373
-
 ATP pH 7.5, 65°C, mutant E114A Pyrococcus horikoshii
0.383
-
 ATP pH 7.5, 65°C, mutant T101A Pyrococcus horikoshii
0.472
-
 ATP pH 7.5, 65°C, mutant W103A Pyrococcus horikoshii
0.531
-
 ATP pH 7.5, 65°C, mutant K100A Pyrococcus horikoshii
0.537
-
 ATP pH 7.5, 65°C, mutant Y105A Pyrococcus horikoshii
0.547
-
 ATP pH 7.5, 65°C, mutant F119A Pyrococcus horikoshii
0.551
-
 ATP pH 7.5, 65°C, mutant D121A Pyrococcus horikoshii
0.578
-
 ATP pH 7.5, 65°C, wild-type enzyme Pyrococcus horikoshii
0.584
-
 ATP pH 7.5, 65°C, mutant K100A/Y105A Pyrococcus horikoshii
0.59 1 ATP pH 7.5, 65°C, mutant Y109A Pyrococcus horikoshii
0.653
-
 ATP pH 8.2, 45°C, mutant W155A Giardia intestinalis
0.675
-
 ATP pH 8.2, 45°C, mutant Q154A Giardia intestinalis
0.683
-
 ATP pH 8.2, 45°C, mutant K170A Giardia intestinalis
0.687
-
 ATP pH 8.2, 45°C, mutant K166A Giardia intestinalis
0.698
-
 ATP pH 8.2, 45°C, mutant K148A Giardia intestinalis
0.711
-
 ATP pH 8.2, 45°C, mutant S153A Giardia intestinalis
0.728
-
 ATP pH 8.2, 45°C, mutant K142A Giardia intestinalis
0.772
-
 ATP pH 8.2, 45°C, mutant K141A Giardia intestinalis
0.796
-
 ATP pH 7.5, 65°C, mutant I104A Pyrococcus horikoshii
0.812
-
 ATP pH 8.2, 45°C, mutant K139A Giardia intestinalis
0.822
-
 ATP pH 8.2, 45°C, mutant K144A Giardia intestinalis
0.834
-
 ATP pH 8.2, 45°C, mutant K152A Giardia intestinalis
0.837
-
 ATP pH 7.5, 65°C, mutant E113A Pyrococcus horikoshii
1.025
-
 ATP pH 8.2, 45°C, mutant D173A Giardia intestinalis
1.157
-
 ATP pH 7.5, 65°C, mutant T118A Pyrococcus horikoshii
1.169
-
 ATP pH 8.2, 45°C, mutant E165A Giardia intestinalis
2.129
-
 ATP pH 8.2, 45°C, mutant E167A Giardia intestinalis
2.177
-
 ATP pH 7.5, 65°C, mutant I115A Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Giardia intestinalis
Mg2+ required Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Giardia intestinalis
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu Pyrococcus horikoshii
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
-
-
Pyrococcus horikoshii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged GlLeuRS from Escherichia coli strain BL21-Codon Plus(DE3)-RIL by nickel affinity chromatography Giardia intestinalis
recombinant His-tagged PhLeuRS from Escherichia coli strain BL21-Codon Plus(DE3)-RIL by nickel affinity chromatography Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
 Giardia intestinalis AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu
-
 Pyrococcus horikoshii AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
additional information measurement of ATP-PPi exchange activity by wild-type and mutant enzymes Giardia intestinalis ?
-
 ?
additional information measurement of ATP-PPi exchange activity by wild-type and mutant enzymes Pyrococcus horikoshii ?
-
 ?

Subunits

Subunits Comment Organism
More three-dimensional LeuRS structure and sequence alignment of different eukaryal and archaeal LSD1s, overview Giardia intestinalis
More three-dimensional LeuRS structure and sequence alignment of different eukaryal and archaeal LSD1s, overview Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
Leucyl-tRNA synthetase
-
 Giardia intestinalis
Leucyl-tRNA synthetase
-
 Pyrococcus horikoshii
LeuRS
-
 Giardia intestinalis
LeuRS
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Giardia intestinalis
65
-
 assay at Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 tRNALeu pH 8.2, 45°C, mutant K142A Giardia intestinalis
0.2
-
 tRNALeu pH 8.2, 45°C, mutant K144A Giardia intestinalis
0.8
-
 tRNALeu pH 8.2, 45°C, mutant D173A Giardia intestinalis
0.8
-
 tRNALeu pH 8.2, 45°C, mutant K141A Giardia intestinalis
0.8
-
 tRNALeu pH 8.2, 45°C, mutant Q154A Giardia intestinalis
1.1
-
 tRNALeu pH 8.2, 45°C, mutant S153A Giardia intestinalis
1.2
-
 tRNALeu pH 8.2, 45°C, mutant E167A Giardia intestinalis
1.3
-
 tRNALeu pH 8.2, 45°C, mutant K152A Giardia intestinalis
1.4
-
 tRNALeu pH 8.2, 45°C, mutant K170A Giardia intestinalis
1.5
-
 tRNALeu pH 8.2, 45°C, mutant K139A Giardia intestinalis
1.9
-
 tRNALeu pH 8.2, 45°C, mutant K148A Giardia intestinalis
2.5
-
 tRNALeu pH 8.2, 45°C, mutant W155A Giardia intestinalis
2.8
-
 tRNALeu pH 8.2, 45°C, mutant E165A Giardia intestinalis
2.8
-
 tRNALeu pH 8.2, 45°C, mutant K166A Giardia intestinalis
3
-
 ATP pH 7.5, 65°C, mutant K100A/Y105A Pyrococcus horikoshii
3.1
-
 L-leucine pH 7.5, 65°C, mutant E114A Pyrococcus horikoshii
3.1
-
 L-leucine pH 7.5, 65°C, mutant K100A/Y105A Pyrococcus horikoshii
3.2
-
 L-leucine pH 7.5, 65°C, mutant D98A Pyrococcus horikoshii
3.4
-
 L-leucine pH 7.5, 65°C, mutant F119A Pyrococcus horikoshii
3.8
-
 L-leucine pH 7.5, 65°C, mutant T101A Pyrococcus horikoshii
3.9
-
 L-leucine pH 7.5, 65°C, mutant K100A Pyrococcus horikoshii
4
-
 ATP pH 7.5, 65°C, mutant F119A Pyrococcus horikoshii
4.1
-
 L-leucine pH 7.5, 65°C, mutant V108A Pyrococcus horikoshii
4.2
-
 ATP pH 7.5, 65°C, mutant V108A Pyrococcus horikoshii
4.3
-
 ATP pH 7.5, 65°C, mutant D98A Pyrococcus horikoshii
4.3
-
 ATP pH 7.5, 65°C, mutant E114A Pyrococcus horikoshii
4.3
-
 L-leucine pH 7.5, 65°C, mutant Y105A Pyrococcus horikoshii
4.4
-
 L-leucine pH 7.5, 65°C, mutant N96A Pyrococcus horikoshii
4.6
-
 ATP pH 7.5, 65°C, mutant K100A Pyrococcus horikoshii
4.6
-
 L-leucine pH 7.5, 65°C, mutant K100A/Y109A Pyrococcus horikoshii
4.7
-
 ATP pH 7.5, 65°C, mutant D121A Pyrococcus horikoshii
4.7
-
 L-leucine pH 7.5, 65°C, mutant R106A Pyrococcus horikoshii
4.7
-
 L-leucine pH 7.5, 65°C, mutant Y109A Pyrococcus horikoshii
4.8
-
 ATP pH 7.5, 65°C, mutant Y109A Pyrococcus horikoshii
4.9
-
 ATP pH 7.5, 65°C, mutant K100A/Y109A Pyrococcus horikoshii
5
-
 ATP pH 7.5, 65°C, mutant T101A Pyrococcus horikoshii
5.1
-
 ATP pH 7.5, 65°C, mutant N96A Pyrococcus horikoshii
5.1
-
 L-leucine pH 7.5, 65°C, mutant R97A Pyrococcus horikoshii
5.1
-
 L-leucine pH 7.5, 65°C, mutant W103A Pyrococcus horikoshii
5.2
-
 L-leucine pH 7.5, 65°C, mutant E113A Pyrococcus horikoshii
5.4
-
 ATP pH 7.5, 65°C, mutant R106A Pyrococcus horikoshii
5.5
-
 ATP pH 7.5, 65°C, mutant R97A Pyrococcus horikoshii
5.6
-
 L-leucine pH 7.5, 65°C, mutant I104A Pyrococcus horikoshii
5.8
-
 ATP pH 7.5, 65°C, mutant E113A Pyrococcus horikoshii
5.9
-
 L-leucine pH 8.2, 45°C, mutant Q154A Giardia intestinalis
6
-
 L-leucine pH 7.5, 65°C, wild-type enzyme Pyrococcus horikoshii
6
-
 L-leucine pH 7.5, 65°C, mutant D121A Pyrococcus horikoshii
6.2
-
 L-leucine pH 8.2, 45°C, mutant K152A Giardia intestinalis
6.5
-
 ATP pH 7.5, 65°C, mutant Y105A Pyrococcus horikoshii
6.8
-
 ATP pH 7.5, 65°C, wild-type enzyme Pyrococcus horikoshii
7.4
-
 ATP pH 7.5, 65°C, mutant I104A Pyrococcus horikoshii
7.4
-
 ATP pH 7.5, 65°C, mutant W103A Pyrococcus horikoshii
7.5
-
 ATP pH 8.2, 45°C, mutant Q154A Giardia intestinalis
7.7
-
 ATP pH 7.5, 65°C, mutant T118A Pyrococcus horikoshii
8.1
-
 L-leucine pH 7.5, 65°C, mutant T118A Pyrococcus horikoshii
8.1
-
 ATP pH 8.2, 45°C, mutant K152A Giardia intestinalis
10.7
-
 L-leucine pH 7.5, 65°C, mutant I115A Pyrococcus horikoshii
13.8
-
 ATP pH 7.5, 65°C, mutant I115A Pyrococcus horikoshii
28.1
-
 L-leucine pH 8.2, 45°C, mutant K170A Giardia intestinalis
28.4
-
 L-leucine pH 8.2, 45°C, mutant K148A Giardia intestinalis
28.7
-
 L-leucine pH 8.2, 45°C, mutant K166A Giardia intestinalis
28.9
-
 L-leucine pH 8.2, 45°C, mutant K144A Giardia intestinalis
29.7
-
 L-leucine pH 8.2, 45°C, mutant E165A Giardia intestinalis
29.8
-
 ATP pH 8.2, 45°C, mutant W155A Giardia intestinalis
29.9
-
 L-leucine pH 8.2, 45°C, mutant K139A Giardia intestinalis
30.1
-
 ATP pH 8.2, 45°C, mutant S153A Giardia intestinalis
31
-
 ATP pH 8.2, 45°C, mutant K170A Giardia intestinalis
31.3
-
 ATP pH 8.2, 45°C, mutant E165A Giardia intestinalis
31.5
-
 ATP pH 8.2, 45°C, mutant K141A Giardia intestinalis
31.7
-
 L-leucine pH 8.2, 45°C, mutant K142A Giardia intestinalis
31.8
-
 ATP pH 8.2, 45°C, mutant K144A Giardia intestinalis
31.9
-
 L-leucine pH 8.2, 45°C, mutant W155A Giardia intestinalis
32.1
-
 ATP pH 8.2, 45°C, mutant K148A Giardia intestinalis
32.3
-
 ATP pH 8.2, 45°C, mutant K166A Giardia intestinalis
32.5
-
 L-leucine pH 8.2, 45°C, mutant S153A Giardia intestinalis
33.1
-
 L-leucine pH 8.2, 45°C, mutant K141A Giardia intestinalis
33.2
-
 ATP pH 8.2, 45°C, mutant K139A Giardia intestinalis
34.4
-
 ATP pH 8.2, 45°C, mutant D173A Giardia intestinalis
36.2
-
 ATP pH 8.2, 45°C, mutant K142A Giardia intestinalis
36.3
-
 L-leucine pH 8.2, 45°C, mutant D173A Giardia intestinalis
54.6
-
 L-leucine pH 8.2, 45°C, mutant E167A Giardia intestinalis
60.2
-
 ATP pH 8.2, 45°C, mutant E167A Giardia intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pyrococcus horikoshii
8.2
-
 assay at Giardia intestinalis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Giardia intestinalis
ATP
-
 Pyrococcus horikoshii

General Information

General Information Comment Organism
evolution the family of leucyl-tRNA synthetases is divided into prokaryotic and eukaryal/archaeal groups according to the presence and position of specific insertions and extensions. e.g. the LSD1, i.e. leucine-specific domain 1, which is naturally present in eukaryal/archaeal LeuRSs, but absent from prokaryotic LeuRSs. The LSD1s from organisms of both groups are dispensable for post-transfer editing Giardia intestinalis
evolution the family of leucyl-tRNA synthetases is divided into prokaryotic and eukaryal/archaeal groups according to the presence and position of specific insertions and extensions. e.g. the LSD1, i.e. leucine-specific domain 1, which is naturally present in eukaryal/archaeal LeuRSs, but absent from prokaryotic LeuRSs. The LSD1s from organisms of both groups are dispensable for post-transfer editing Pyrococcus horikoshii
additional information leucine-specific domain 1, LSD1, is dispensable for post-transfer editing Giardia intestinalis
additional information leucine-specific domain 1, LSD1, is dispensable for post-transfer editing Pyrococcus horikoshii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.2
-
 ATP pH 7.5, 65°C, mutant K100A/Y105A Pyrococcus horikoshii
6.4
-
 ATP pH 7.5, 65°C, mutant I115A Pyrococcus horikoshii
6.6
-
 ATP pH 7.5, 65°C, mutant T118A Pyrococcus horikoshii
6.9
-
 ATP pH 7.5, 65°C, mutant E113A Pyrococcus horikoshii
7.3
-
 ATP pH 7.5, 65°C, mutant F119A Pyrococcus horikoshii
8.1
-
 ATP pH 7.5, 65°C, mutant Y109A Pyrococcus horikoshii
8.5
-
 ATP pH 7.5, 65°C, mutant D121A Pyrococcus horikoshii
8.7
-
 ATP pH 7.5, 65°C, mutant K100A Pyrococcus horikoshii
9.3
-
 ATP pH 7.5, 65°C, mutant I104A Pyrococcus horikoshii
9.7
-
 ATP pH 8.2, 45°C, mutant K152A Giardia intestinalis
11.1
-
 ATP pH 8.2, 45°C, mutant Q154A Giardia intestinalis
11.5
-
 ATP pH 7.5, 65°C, mutant E114A Pyrococcus horikoshii
11.8
-
 ATP pH 7.5, 65°C, wild-type enzyme Pyrococcus horikoshii
11.9
-
 ATP pH 7.5, 65°C, mutant D98A Pyrococcus horikoshii
12
-
 ATP pH 7.5, 65°C, mutant V108A Pyrococcus horikoshii
12.1
-
 ATP pH 7.5, 65°C, mutant Y105A Pyrococcus horikoshii
13.1
-
 ATP pH 7.5, 65°C, mutant T101A Pyrococcus horikoshii
13.5
-
 ATP pH 7.5, 65°C, mutant K100A/Y109A Pyrococcus horikoshii
13.8
-
 ATP pH 7.5, 65°C, mutant N96A Pyrococcus horikoshii
15.7
-
 ATP pH 7.5, 65°C, mutant W103A Pyrococcus horikoshii
17
-
 ATP pH 7.5, 65°C, mutant R97A Pyrococcus horikoshii
18.2
-
 ATP pH 7.5, 65°C, mutant R106A Pyrococcus horikoshii
26.8
-
 ATP pH 8.2, 45°C, mutant E165A Giardia intestinalis
28.3
-
 ATP pH 8.2, 45°C, mutant E167A Giardia intestinalis
33.5
-
 ATP pH 8.2, 45°C, mutant D173A Giardia intestinalis
38
-
 tRNALeu pH 8.2, 45°C, mutant K144A Giardia intestinalis
38.7
-
 ATP pH 8.2, 45°C, mutant K144A Giardia intestinalis
40.8
-
 ATP pH 8.2, 45°C, mutant K141A Giardia intestinalis
40.9
-
 ATP pH 8.2, 45°C, mutant K139A Giardia intestinalis
42.3
-
 ATP pH 8.2, 45°C, mutant S153A Giardia intestinalis
45.4
-
 ATP pH 8.2, 45°C, mutant K170A Giardia intestinalis
45.6
-
 ATP pH 8.2, 45°C, mutant W155A Giardia intestinalis
46
-
 ATP pH 8.2, 45°C, mutant K148A Giardia intestinalis
47
-
 ATP pH 8.2, 45°C, mutant K166A Giardia intestinalis
49.7
-
 ATP pH 8.2, 45°C, mutant K142A Giardia intestinalis
57
-
 tRNALeu pH 8.2, 45°C, mutant K142A Giardia intestinalis
132
-
 tRNALeu pH 8.2, 45°C, mutant K139A Giardia intestinalis
136
-
 tRNALeu pH 8.2, 45°C, mutant D173A Giardia intestinalis
157
-
 tRNALeu pH 8.2, 45°C, mutant K141A Giardia intestinalis
210
-
 tRNALeu pH 8.2, 45°C, mutant Q154A Giardia intestinalis
245
-
 tRNALeu pH 8.2, 45°C, mutant E167A Giardia intestinalis
255
-
 tRNALeu pH 8.2, 45°C, mutant K152A Giardia intestinalis
275
-
 tRNALeu pH 8.2, 45°C, mutant K170A Giardia intestinalis
275
-
 tRNALeu pH 8.2, 45°C, mutant S153A Giardia intestinalis
286
-
 L-leucine pH 7.5, 65°C, mutant F119A Pyrococcus horikoshii
337
-
 tRNALeu pH 8.2, 45°C, mutant E165A Giardia intestinalis
346
-
 tRNALeu pH 8.2, 45°C, mutant K166A Giardia intestinalis
352
-
 tRNALeu pH 8.2, 45°C, mutant W155A Giardia intestinalis
380
-
 tRNALeu pH 8.2, 45°C, mutant K148A Giardia intestinalis
386
-
 L-leucine pH 7.5, 65°C, mutant T101A Pyrococcus horikoshii
438
-
 L-leucine pH 7.5, 65°C, mutant K100A Pyrococcus horikoshii
493
-
 L-leucine pH 7.5, 65°C, mutant K100A/Y105A Pyrococcus horikoshii
498
-
 L-leucine pH 7.5, 65°C, mutant E114A Pyrococcus horikoshii
521
-
 L-leucine pH 7.5, 65°C, mutant Y109A Pyrococcus horikoshii
541
-
 L-leucine pH 7.5, 65°C, mutant D98A Pyrococcus horikoshii
550
-
 L-leucine pH 7.5, 65°C, mutant R106A Pyrococcus horikoshii
550
-
 L-leucine pH 7.5, 65°C, mutant R97A Pyrococcus horikoshii
586
-
 L-leucine pH 7.5, 65°C, mutant W103A Pyrococcus horikoshii
706
-
 L-leucine pH 7.5, 65°C, mutant V108A Pyrococcus horikoshii
775
-
 L-leucine pH 7.5, 65°C, mutant Y105A Pyrococcus horikoshii
806
-
 L-leucine pH 7.5, 65°C, mutant K100A/Y109A Pyrococcus horikoshii
819
-
 L-leucine pH 7.5, 65°C, mutant N96A Pyrococcus horikoshii
839
-
 L-leucine pH 7.5, 65°C, mutant D121A Pyrococcus horikoshii
846
-
 L-leucine pH 7.5, 65°C, mutant E113A Pyrococcus horikoshii
984
-
 L-leucine pH 7.5, 65°C, mutant I104A Pyrococcus horikoshii
1013
-
 L-leucine pH 7.5, 65°C, wild-type enzyme Pyrococcus horikoshii
1277
-
 L-leucine pH 7.5, 65°C, mutant T118A Pyrococcus horikoshii
1686
-
 L-leucine pH 8.2, 45°C, mutant Q154A Giardia intestinalis
1889
-
 L-leucine pH 8.2, 45°C, mutant K144A Giardia intestinalis
1892
-
 L-leucine pH 8.2, 45°C, mutant E165A Giardia intestinalis
1910
-
 L-leucine pH 7.5, 65°C, mutant I115A Pyrococcus horikoshii
1952
-
 L-leucine pH 8.2, 45°C, mutant K166A Giardia intestinalis
1980
-
 L-leucine pH 8.2, 45°C, mutant K139A Giardia intestinalis
1993
-
 L-leucine pH 8.2, 45°C, mutant K170A Giardia intestinalis
2044
-
 L-leucine pH 8.2, 45°C, mutant S153A Giardia intestinalis
2186
-
 L-leucine pH 8.2, 45°C, mutant K142A Giardia intestinalis
2200
-
 L-leucine pH 8.2, 45°C, mutant D173A Giardia intestinalis
2290
-
 L-leucine pH 8.2, 45°C, mutant K148A Giardia intestinalis
2508
-
 L-leucine pH 8.2, 45°C, mutant K141A Giardia intestinalis
2583
-
 L-leucine pH 8.2, 45°C, mutant K152A Giardia intestinalis
2681
-
 L-leucine pH 8.2, 45°C, mutant W155A Giardia intestinalis
3370
-
 L-leucine pH 8.2, 45°C, mutant E167A Giardia intestinalis