Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Homo sapiens |
expressed in Escherichia coli as a His-tagged fusion protein | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D399K | mutant is resitant to inhibitor 5-fluoro-2,1-benzoxaborol-1(3H)-ol but more sensitive to norvaline inhibition | Homo sapiens |
D418R | mutant is significantly resistant to inhibitor AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Growth rate is moderately inhibited in medium containing a large excess of norvaline and reduced leucine | Saccharomyces cerevisiae |
D419A | mutant is significantly resistant to inhibitor AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Mutant D419A shows mischarging capacity with Ile. Growth rate is severly inhibited in medium containing a large excess of norvaline and reduced leucine | Saccharomyces cerevisiae |
DELTA270-530 | deletion of the CP1 domain shows that the mutant is not able to rescue LeuRS knock-out strain | Saccharomyces cerevisiae |
DELTA314-319 | deletion of the T-rich region shows that the mutant is able to rescue LeuRS knock-out strain with a grwoth rate similar to wild-type | Saccharomyces cerevisiae |
K404Y | mutant is significantly resistant to inhibitor AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Growth rate is moderately inhibited in medium containing a large excess of norvaline and reduced leucine | Saccharomyces cerevisiae |
additional information | deletion of the leuS gene is lethal. Human cytoplasmic LeuRS can rescue the knock-out strain but not Escherichia coli LeuRS. LeuRS mutant strains T319, D419A, K404Y, S416D, D418R, T347A or T410A are also able to rescue the null mutant | Saccharomyces cerevisiae |
additional information | growth rate of wild-type strain in media containing a large excess of noncognate amino acid norvaline and reduced leucine is reduced to 35% | Saccharomyces cerevisiae |
S416D | mutant is significantly resistant to inhibitor AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Growth rate is moderately inhibited in medium containing a large excess of norvaline and reduced leucine | Saccharomyces cerevisiae |
T31E | mutant is significantly resistant to inhibitor AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Mutant T319A shows mischarging capacity with Ile. Growth rate is severly inhibited in medium containing a large excess of norvaline and reduced leucine | Saccharomyces cerevisiae |
T347A | mutant is inhibited by AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Growth rate is moderately inhibited in medium containing a large excess of norvaline and reduced leucine | Saccharomyces cerevisiae |
T410A | mutant is inhibited by AN-2690. Mutant aminoacylation of yctRNALeu is not different from wild-type ycLeuRS. Growth rate is similar to wild-type. Growth rate is moderately inhibited in medium containing a large excess of norvaline and reduced leucine | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-2,1-benzoxaborol-1(3H)-ol | AN-2690, antibiotic which specifically targets the editing active site of LeuRS | Homo sapiens | |
5-fluoro-2,1-benzoxaborol-1(3H)-ol | AN-2690, antibiotic which specifically targets the editing active site of LeuRS | Saccharomyces cerevisiae | |
norvaline | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
L-leucine | - |
Saccharomyces cerevisiae | |
0.167 | - |
norvaline | - |
Saccharomyces cerevisiae | |
8.713 | - |
L-isoleucine | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | P26637 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Homo sapiens |
using Ni-NTA chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNALeu | - |
Homo sapiens | AMP + diphosphate + L-isoleucyl-tRNALeu | - |
? | |
ATP + L-isoleucine + tRNALeu | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-isoleucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-norvaline + tRNALeu | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-norvalyl-tRNALeu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HcleuRS | - |
Homo sapiens |
Leucyl-tRNA synthetase | - |
Homo sapiens |
leucyltRNA synthetase | - |
Saccharomyces cerevisiae |
LeuRS | - |
Homo sapiens |
LeuRS | - |
Saccharomyces cerevisiae |
ycLeuRS | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.2 | - |
L-isoleucine | - |
Saccharomyces cerevisiae | |
13.5 | - |
norvaline | - |
Saccharomyces cerevisiae | |
37.5 | - |
L-leucine | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Saccharomyces cerevisiae |