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Literature summary for 6.1.1.4 extracted from

  • Nawaz, M.H.; Pang, Y.L.; Martinis, S.A.
    Molecular and functional dissection of a putative RNA-binding region in yeast mitochondrial leucyl-tRNA synthetase (2007), J. Mol. Biol., 367, 384-394.
    View publication on PubMed
Search for more literature for 6.1.1.4

Protein Variants

Protein Variants Comment Organism
R449A site-directed mutagenesis, nearly inactive mutant Saccharomyces cerevisiae
R449E site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320 Saccharomyces cerevisiae
R449K site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320, 30fold reduced activity compared to the wild-type enzyme Saccharomyces cerevisiae
R451A site-directed mutagenesis, nearly inactive mutant Saccharomyces cerevisiae
R451E site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320 Saccharomyces cerevisiae
R451K site-directed mutagenesis, mutation within the RDW peptide, complementation of the null mutant strain QBY320, 11fold reduced activity compared to the wild-type enzyme Saccharomyces cerevisiae
W445A site-directed mutagenesis, nearly inactive mutant Saccharomyces cerevisiae
W445F site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320 Saccharomyces cerevisiae
W445H site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320 Saccharomyces cerevisiae
W445K site-directed mutagenesis, mutation within the RDW peptide, no complementation of the null mutant strain QBY320 Saccharomyces cerevisiae
W445Y site-directed mutagenesis, mutation within the RDW peptide, weak complementation of the null mutant strain QBY320, 30fold reduced activity compared to the wild-type enzyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of recombinant wild-type and mutant enzymes Saccharomyces cerevisiae
0.0002
-
 tRNALeu mutant R449K Saccharomyces cerevisiae
0.0005
-
 tRNALeu mutant W445Y Saccharomyces cerevisiae
0.0006
-
 tRNALeu wild-type enzyme Saccharomyces cerevisiae
0.0007
-
 tRNALeu mutant R451K Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-leucine + tRNALeu Saccharomyces cerevisiae
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
 Saccharomyces cerevisiae AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu a two step reaction, the first of which is reversible, aminoacylation and editing by LeuRS require migration of the tRNA acceptor stem end between the canonical aminoacylation core and a separate domain called CP1 that is responsible for amino acid editing, post-transfer editing mechanism., overview Saccharomyces cerevisiae AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
additional information the LeuRS CP1 domain can also support group I intron RNA splicing in the yeast mitochondria, overview, the RDW peptide, a highly conserved peptide within an RDW-containing motif, is important for enzyme interactions, the RDW peptide is dynamic and forms unique sets of interactions with the aminoacylation and editing complexes, overview Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
More primary and tertiary structure, overview, the RDW peptide, a highly conserved peptide within an RDW-containing motif, is important for enzyme interactions, overview Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Leucyl-tRNA synthetase
-
 Saccharomyces cerevisiae
LeuRS
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 tRNALeu mutant R449K Saccharomyces cerevisiae
0.2
-
 tRNALeu mutant W445Y Saccharomyces cerevisiae
0.6
-
 tRNALeu mutant R451K Saccharomyces cerevisiae
5
-
 tRNALeu wild-type enzyme Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae