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Literature summary for 6.1.1.4 extracted from
- Functional association between three archaeal aminoacyl-tRNA synthetases (2007), J. Biol. Chem., 282, 3680-3687.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | a complex between prolyl-tRNA synthetase, ProRS, and LeuRS in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview | Methanothermobacter thermautotrophicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of prolyl-tRNA synthetase, ProRS, and LeuRS in a Saccharomyces cerevisiae two-hybrid system, expression of His6-tagged LeuRS in Escherichia coli strain BL21(DE3) | Methanothermobacter thermautotrophicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | prolyl-tRNA synthetase, ProRS, and LeuRS interaction kinetics | Methanothermobacter thermautotrophicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Methanothermobacter thermautotrophicus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | Methanothermobacter thermautotrophicus | a complex between prolyl-tRNA synthetase, ProRS, and LeuRS in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged LeuRS from Escherichia coli strain BL21(DE3) by anion exchange chromatography, gel filtration, and ultrafiltration, co-purification of prolyl-tRNA synthetase, ProRS, and LeuRS, overview | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
| ATP + L-leucine + tRNALeu | a complex between prolyl-tRNA synthetase, ProRS, and LeuRS in Methanothermobacter thermautotrophicus enhances tRNAPro aminoacylation, overview | Methanothermobacter thermautotrophicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Leucyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
| LeuRS | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Methanothermobacter thermautotrophicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Methanothermobacter thermautotrophicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Methanothermobacter thermautotrophicus | |
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