Cloned (Comment) | Organism |
---|---|
2 genes leuS and leuS', overexpression in Escherichia coli BL21(DE3) | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
tRNALeu from Escherichia coli | recombinant enzyme complex, 55°C | Aquifex aeolicus | |
0.0014 | - |
tRNALeu from Aquifex aeolicus | recombinant enzyme complex, 55°C | Aquifex aeolicus | |
0.0083 | - |
L-leucine | recombinant enzyme complex, 65°C | Aquifex aeolicus | |
0.228 | - |
ATP | recombinant enzyme complex, 65°C | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
heterodimer in quartenary structure, 2 * 35000, leuS gene product, + 2 * 65000, leuS gene product, SDS-PAGE | Aquifex aeolicus |
65000 | - |
heterodimer in quartenary structure, 2 * 35000, leuS gene product, + 2 * 65000, leuS gene product, SDS-PAGE | Aquifex aeolicus |
100000 | - |
complexed recombinant leuS' and leuS gene product, gel filtration | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | Aquifex aeolicus | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
class I enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzymes from Escherichia coli, leuS' gene product to homogeneity | Aquifex aeolicus |
Renatured (Comment) | Organism |
---|---|
renaturation and refolding of recombinant leuS gene product expressed in Escherichia coli | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | enzyme activity only appears when both gene products, of leuS and leuS', coexist | Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu from Aquifex aeolicus | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu from Escherichia coli | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | heterodimer in quartenary structure, 2 * 35000, leuS' gene product, + 2 * 65000, leuS gene product, SDS-PAGE | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
Leucine translase | - |
Aquifex aeolicus |
Leucine--tRNA ligase | - |
Aquifex aeolicus |
Leucyl-transfer ribonucleate synthetase | - |
Aquifex aeolicus |
Leucyl-transfer ribonucleic acid synthetase | - |
Aquifex aeolicus |
Leucyl-transfer RNA synthetase | - |
Aquifex aeolicus |
Leucyl-tRNA synthetase | - |
Aquifex aeolicus |
LeuRS | - |
Aquifex aeolicus |
Synthetase, leucyl-transfer ribonucleate | - |
Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
tRNALeu from Escherichia coli | recombinant enzyme complex, 55°C | Aquifex aeolicus | |
0.006 | - |
tRNALeu from Aquifex aeolicus | recombinant enzyme complex, 55°C | Aquifex aeolicus | |
0.094 | - |
ATP | recombinant enzyme complex, 65°C | Aquifex aeolicus | |
0.134 | - |
L-leucine | recombinant enzyme complex, 65°C | Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Aquifex aeolicus |