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Literature summary for 6.1.1.4 extracted from
- Expression and characterization of the human mitochondrial leucyl-tRNA synthetase (2000), Biochim. Biophys. Acta, 1490, 245-258.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mitochondrial isozyme, DNA sequence determination and analysis, expression of the mature enzyme as His-tagged enzyme in Escherichia coli BL21(DE3) | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inhibition by high levels of mono- and divalent cations | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
tRNALeu | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens |  |
| 0.045 | - |
L-leucine | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens | |
| 0.09 | - |
ATP | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | mitochondrial isozyme | Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 101000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | Homo sapiens | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q15031 | class I enzyme | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | - |
Homo sapiens | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
| ATP + L-leucine + tRNALeu | tRNALeu substrate from Escherichia coli, 2-step reaction, the first step is reversible, while the second step is not | Homo sapiens | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
| additional information | enzyme also performs the ATP-diphosphate exchange reaction | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 101000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Leucine translase | - |
Homo sapiens |
| Leucine--tRNA ligase | - |
Homo sapiens |
| Leucyl-transfer ribonucleate synthetase | - |
Homo sapiens |
| Leucyl-transfer ribonucleic acid synthetase | - |
Homo sapiens |
| Leucyl-transfer RNA synthetase | - |
Homo sapiens |
| Leucyl-tRNA synthetase | - |
Homo sapiens |
| LeuRS | - |
Homo sapiens |
| More | enzyme belongs to the XUX aminoacyl synthetase family | Homo sapiens |
| mtLeuRS | - |
Homo sapiens |
| Synthetase, leucyl-transfer ribonucleate | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
tRNALeu | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens | |
| 0.8 | - |
ATP | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens | |
| 2.7 | - |
L-leucine | recombinant mitochondrial isozyme, pH 7.6, 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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