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Literature summary for 6.1.1.4 extracted from
- Chloroplast leucyl-tRNA synthetase from Euglena gracilis. Purification, kinetic analysis, and structural characterization (1981), Biochemistry, 20, 5855-5859.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
tRNALeu | - |
Euglena gracilis |  |
| 0.008 | - |
L-Leu | - |
Euglena gracilis | |
| 0.13 | - |
ATP | - |
Euglena gracilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Euglena gracilis | 9507 | - |
| cytoplasm | - |
Euglena gracilis | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme) | Euglena gracilis | |
| Ca2+ | 75% of the maximal activity obtained with Mg2+ | Euglena gracilis | |
| Co2+ | can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme | Euglena gracilis | |
| Co2+ | 40% of the maximal activity obtained with Mg2+ | Euglena gracilis | |
| Mg2+ | activates | Euglena gracilis | |
| Mg2+ | optimal Mg2+/ATP ratio: 1.2 | Euglena gracilis | |
| Mn2+ | can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme) | Euglena gracilis | |
| Mn2+ | 75% of the maximal activity obtained with Mg2+ | Euglena gracilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
gel filtration | Euglena gracilis |
| 116000 | - |
gel filtration | Euglena gracilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.233 | - |
- |
Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | - |
Euglena gracilis | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 100000, urea-SDS-PAGE | Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Euglena gracilis |
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