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Literature summary for 6.1.1.3 extracted from

  • Ling, J.; Peterson, K.M.; Simonovic, I.; Cho, C.; Soell, D.; Simonovic, M.
    Yeast mitochondrial threonyl-tRNA synthetase recognizes tRNA isoacceptors by distinct mechanisms and promotes CUN codon reassignment (2012), Proc. Natl. Acad. Sci. USA, 109, 3281-3286.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Codon plus cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D423A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
D437A the mutant shows strongly increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
E401A the mutant shows strongly increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
K408A the mutant shows about wild type catalytic efficiency for tRNAThr1 Saccharomyces cerevisiae
K440A the mutant shows increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
N356A the mutant shows increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
N359A the mutant shows increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
N400A the mutant shows increased catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
N432A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
N432A the mutant shows weaker binding for tRNAThr1 and displays increased Km for tRNAThr2 as well Saccharomyces cerevisiae
Q362A the mutant displays increased Km values for tRNAThr2 Saccharomyces cerevisiae
R434A the mutant shows weaker binding for tRNAThr1 only Saccharomyces cerevisiae
R439A the mutant shows weaker binding for tRNAThr1 and displays increased Km for tRNAThr2 as well Saccharomyces cerevisiae
S409E the mutant shows about wild type catalytic efficiency for tRNAThr1 Saccharomyces cerevisiae
T357A the mutant displays increased Km values for tRNAThr2 Saccharomyces cerevisiae
Y405A the mutant displays increased Km values for tRNAThr2 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00013
-
tRNA1Thr mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.0002
-
tRNA1Thr mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00024
-
tRNA1Thr mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00024
-
tRNA1Thr mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00027
-
tRNA2Thr mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00028
-
tRNA1Thr mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00029
-
tRNA1Thr wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.0003
-
tRNA1Thr mutant enzyme E405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.0003
-
tRNA1Thr mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00031
-
tRNA1Thr mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00031
-
tRNA1Thr mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00037
-
tRNA2Thr mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00044
-
tRNA2Thr mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00044
-
tRNA2Thr wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00046
-
tRNA1Thr mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00047
-
tRNA1Thr mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00048
-
tRNA1Thr mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00049
-
tRNA1Thr mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00049
-
tRNA2Thr mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00051
-
tRNA2Thr mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00059
-
tRNA2Thr mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00059
-
tRNA1Thr mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00064
-
tRNA2Thr mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00069
-
tRNA2Thr mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00078
-
tRNA2Thr mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00083
-
tRNA1Thr mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00083
-
tRNA2Thr mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00094
-
tRNA2Thr mutant enzyme E405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00095
-
tRNA1Thr mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00123
-
tRNA2Thr mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.00139
-
tRNA2Thr mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.0014
-
tRNA2Thr mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains a Zn2+ ion Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-threonine + tRNA1Thr Saccharomyces cerevisiae
-
AMP + diphosphate + L-threonyl-tRNA1Thr
-
?
ATP + L-threonine + tRNA2Thr Saccharomyces cerevisiae
-
AMP + diphosphate + L-threonyl-tRNA2Thr
-
?
ATP + L-threonine + tRNAThr Saccharomyces cerevisiae
-
AMP + diphosphate + L-threonyl-tRNAThr
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-threonine + tRNA1Thr
-
Saccharomyces cerevisiae AMP + diphosphate + L-threonyl-tRNA1Thr
-
?
ATP + L-threonine + tRNA2Thr
-
Saccharomyces cerevisiae AMP + diphosphate + L-threonyl-tRNA2Thr
-
?
ATP + L-threonine + tRNAThr
-
Saccharomyces cerevisiae AMP + diphosphate + L-threonyl-tRNAThr
-
?

Synonyms

Synonyms Comment Organism
Mst1
-
Saccharomyces cerevisiae
Threonyl-tRNA synthetase
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
tRNA2Thr mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.017
-
tRNA2Thr mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.03
-
tRNA2Thr mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.033
-
tRNA1Thr mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.038
-
tRNA2Thr wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.04
-
tRNA2Thr mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.043
-
tRNA2Thr mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.045
-
tRNA2Thr mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.045
-
tRNA2Thr mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.05
-
tRNA1Thr mutant enzyme N400A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.05
-
tRNA1Thr mutant enzyme N432A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.05
-
tRNA1Thr mutant enzyme S409E, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.05
-
tRNA1Thr wild type enzyme, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.053
-
tRNA1Thr mutant enzyme D437A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.053
-
tRNA2Thr mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.055
-
tRNA1Thr mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.058
-
tRNA2Thr mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.06
-
tRNA1Thr mutant enzyme N356A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.06
-
tRNA2Thr mutant enzyme Y405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.067
-
tRNA1Thr mutant enzyme D423A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.067
-
tRNA2Thr mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.073
-
tRNA1Thr mutant enzyme E401A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.08
-
tRNA1Thr mutant enzyme T357A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.082
-
tRNA1Thr mutant enzyme Y405A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.087
-
tRNA1Thr mutant enzyme N359A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.088
-
tRNA2Thr mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.093
-
tRNA1Thr mutant enzyme K408A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.1
-
tRNA2Thr mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.103
-
tRNA2Thr mutant enzyme R434A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.112
-
tRNA1Thr mutant enzyme K440A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.117
-
tRNA1Thr mutant enzyme Q362A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae
0.118
-
tRNA1Thr mutant enzyme R439A, in 100 mM Na-HEPES pH 7.2, 30 mM KCl, 10 mM MgCl2C, temperature not specified in the publication Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae