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Literature summary for 6.1.1.3 extracted from

  • Su, D.; Lieberman, A.; Lang, B.F.; Simonovic, M.; Soell, D.; Ling, J.
    An unusual tRNAThr derived from tRNAHis reassigns in yeast mitochondria the CUN codons to threonine (2011), Nucleic Acids Res., 39, 4866-4874.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of MST1 with different tRNAs, overview Saccharomyces cerevisiae
0.00029
-
 tRNAThr pH 7.2, 37°C, tRNAThr1, MST1 Saccharomyces cerevisiae
0.00044
-
 tRNAThr pH 7.2, 37°C, tRNAThr2, MST1 Saccharomyces cerevisiae
0.0015
-
 tRNAThr pH 7.2, 37°C, tRNAThr1(G-1), MST1 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae
Mg2+
-
 Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-threonine + tRNAThr Saccharomyces cerevisiae
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
ATP + L-threonine + tRNAThr Candida albicans
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-threonine + tRNAThr
-
 Saccharomyces cerevisiae AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
ATP + L-threonine + tRNAThr
-
 Candida albicans AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
ATP + L-threonine + tRNAThr MST1 can attach threonine to both tRNAThr1 and the regular tRNAThr2, but not to the wild-type tRNAHis. But a loss of the first nucleotide G-1 in tRNAHis converts it to a substrate for MST1 Saccharomyces cerevisiae AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
additional information addition of first nucleotide G-1 to tRNAThr1 allows efficient histidylation by histidyl-tRNA synthetase Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
mitochondrial threonyl-tRNA synthetase
-
 Saccharomyces cerevisiae
mitochondrial threonyl-tRNA synthetase
-
 Candida albicans
Mst1
-
 Saccharomyces cerevisiae
Mst1
-
 Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae
37
-
 assay at Candida albicans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00157
-
 tRNAThr pH 7.2, 37°C, tRNAThr1(G-1), MST1 Saccharomyces cerevisiae
0.0383
-
 tRNAThr pH 7.2, 37°C, tRNAThr2, MST1 Saccharomyces cerevisiae
0.0467
-
 tRNAThr pH 7.2, 37°C, tRNAThr1, MST1 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Saccharomyces cerevisiae
7.2
-
 assay at Candida albicans

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae
ATP
-
 Candida albicans

General Information

General Information Comment Organism
evolution evolutionary development of unusual tRNAThr1, containing an enlarged 8-nt anticodon loop, which is required to reassign CUN codons from leucine to threonine, tRNAThr phylogenetic analysis, overview. MST1 has co-evolved with tRNAThr, which evoled from tRNAHis Saccharomyces cerevisiae
evolution tRNAThr phylogenetic analysis, overview Candida albicans
metabolism in certain yeast mitochondria, CUN codons are reassigned from leucine to threonine, which requires an unusual tRNAThr with an enlarged 8-nt anticodon loop, tRNAThr1 Saccharomyces cerevisiae
metabolism in certain yeast mitochondria, CUN codons are reassigned from leucine to threonine, which requires an unusual tRNAThr with an enlarged 8-nt anticodon loop, tRNAThr1. But in Candida albicans, the CUN codons remain assigned to leucine, and MST1 cannot threonylate tRNAThr Candida albicans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.093
-
 tRNAThr pH 7.2, 37°C, tRNAThr1(G-1), MST1 Saccharomyces cerevisiae
90
-
 tRNAThr pH 7.2, 37°C, tRNAThr2, MST1 Saccharomyces cerevisiae
171.7
-
 tRNAThr pH 7.2, 37°C, tRNAThr1, MST1 Saccharomyces cerevisiae