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Literature summary for 6.1.1.3 extracted from

  • Zheltonosova, J.; Melnikova, E.; Garber, M.; Reinbolt, J.; Kern, D.; Ehresmann, C.; Ehresmann, B.
    Threonyl-tRNA synthetase from Thermus thermophilus. Purification and some structural and kinetic properties (1994), Biochimie, 76, 71-77.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000037 0.00007 tRNAThr of Thermus thermophilus, , depending on temperature Thermus thermophilus
0.00005
-
tRNAThr of E. coli Escherichia coli
0.000085
-
tRNAThr
-
Escherichia coli
0.00014
-
tRNAThr of E. coli Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
2 * 77000, SDS-PAGE under denaturing conditions Thermus thermophilus
150000
-
-
Escherichia coli
150000
-
gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Thermus thermophilus
-
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.353
-
-
Thermus thermophilus

Storage Stability

Storage Stability Organism
4°C Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-threonine + tRNAThr
-
Thermus thermophilus AMP + diphosphate + L-threonyl-tRNAThr
-
?
ATP + L-threonine + tRNAThr
-
Escherichia coli AMP + diphosphate + L-threonyl-tRNAThr
-
?
ATP + L-threonine + tRNAThr tRNAThr of Thermus thermophilus Thermus thermophilus AMP + diphosphate + L-threonyl-tRNAThr
-
?
ATP + L-threonine + tRNAThr tRNAThr3 of Escherichia coli Escherichia coli AMP + diphosphate + L-threonyl-tRNAThr
-
?
ATP + L-threonine + tRNAThr
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-threonyl-tRNAThr
-
?
ATP + L-threonine + tRNAThr tRNAThr of Thermus thermophilus Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-threonyl-tRNAThr
-
?

Subunits

Subunits Comment Organism
dimer
-
Escherichia coli
dimer 2 * 77000, SDS-PAGE under denaturing conditions Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Escherichia coli
70
-
-
Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.037 0.23 tRNAThr
-
Thermus thermophilus
0.21
-
tRNAThr tRNAThr of E. coli Thermus thermophilus
0.52 0.7 tRNAThr tRNAThr of Thermus thermophilus Escherichia coli
0.53
-
tRNAThr tRNAThr of E. coli Escherichia coli