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Literature summary for 6.1.1.27 extracted from

  • Zhang, C.M.; Liu, C.; Slater, S.; Hou, Y.M.
    Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys) (2008), Nat. Struct. Mol. Biol., 15, 507-514.
    View publication on PubMed
Search for more literature for 6.1.1.27

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state and single-turnover kinetics, kinetic analysis, overview Methanocaldococcus jannaschii
0.00097
-
 m1G37-tRNACys 60°C, pH 6.0, steady-state kinetics Methanocaldococcus jannaschii
0.0011
-
 tRNACys 60°C, pH 6.0, steady-state kinetics Methanocaldococcus jannaschii
0.007
-
 tRNACys pH 7.5, 37°C, recombinant enzyme, native tRNACys Methanocaldococcus jannaschii
0.0097
-
 tRNACys pH 7.5, 37°C, recombinant enzyme, m1G37 tRNACys Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + O-phospho-L-serine + tRNACys Methanocaldococcus jannaschii
-
 AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
ATP + O-phospho-L-serine + tRNACys Methanocaldococcus jannaschii Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryl–tRNA synthetase (SepRS) acylates tRNACys with phosphoserine (Sep), and Sep-tRNA–Cys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
additional information Methanocaldococcus jannaschii Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryl-tRNA synthetase acylates tRNACys with phosphoserine, and Sep-tRNA-Cys-tRNA synthase converts the tRNA-bound phosphoserine to cysteine. Methanocaldococcus jannaschii SepRS differs from CysRS by recruiting the m1G37 modification as a determinant for aminoacylation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + O-phospho-L-serine + m1G37-tRNACys
-
 Methanocaldococcus jannaschii AMP + diphosphate + O-phospho-L-seryl-m1G37-tRNACys
-
 ?
ATP + O-phospho-L-serine + tRNACys
-
 Methanocaldococcus jannaschii AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
ATP + O-phospho-L-serine + tRNACys Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryl–tRNA synthetase (SepRS) acylates tRNACys with phosphoserine (Sep), and Sep-tRNA–Cys-tRNA synthase (SepCysS) converts the tRNA-bound phosphoserine to cysteine Methanocaldococcus jannaschii AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
ATP + O-phospho-L-serine + tRNACys SepRS differs from CysRS (EC 6.1.1.16) by recruiting the m1G37 modification as a determinant for aminoacylation, and in showing limited discrimination against mutations of conserved nucleotides. O-Phosphoseryl–tRNA synthetase and Sep-tRNA–Cys-tRNA synthase bind the reaction intermediate O-phospho-L-serine-tRNACys tightly, and these two enzymes form a stable binary complex that promotes conversion of the intermediate to the product and sequesters the intermediate from binding to elongation factor EF-1a or infiltrating into the ribosome Methanocaldococcus jannaschii AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
ATP + O-phospho-L-serine + tRNACys SepRS differs from CysRS by recruiting the m1G37 modification as a determinant for aminoacylation, and in showing limited discrimination against mutations of conserved nucleotides. The enzyme requires the S-adenosylmethione-dependent formation of m1G37 in the anticodon loop for efficient aminoacylation Methanocaldococcus jannaschii AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?
additional information Methanocaldococcus jannaschii synthesizes Cys-tRNACys by an indirect pathway, whereby O-phosphoseryl-tRNA synthetase acylates tRNACys with phosphoserine, and Sep-tRNA-Cys-tRNA synthase converts the tRNA-bound phosphoserine to cysteine. Methanocaldococcus jannaschii SepRS differs from CysRS by recruiting the m1G37 modification as a determinant for aminoacylation Methanocaldococcus jannaschii ?
-
 ?
additional information kinetic and binding measurements show that both SepRS and Sep-tRNA-Cys-tRNA synthase, SepCysS, bind the reaction intermediate Sep-tRNACys tightly, and these two enzymes form a stable binary complex that promotes conversion of the intermediate to the product and sequesters the intermediate from binding to elongation factor EF-1alpha or infiltrating into the ribosome, mechanism of the binary complex, detailed overview Methanocaldococcus jannaschii ?
-
 ?

Subunits

Subunits Comment Organism
More both SepRS and SepCysS are active as a monomer in theSepRS–SepCysS binary complex Methanocaldococcus jannaschii
tetramer SepRS contains two tRNACys molecules per tetramer indicating an asymmetry of the four identical subunits Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
SepRS SepRS–SepCysS binary complex Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 tRNACys 60°C, pH 6.0, steady-state kinetics Methanocaldococcus jannaschii
0.24
-
 m1G37-tRNACys 60°C, pH 6.0, steady-state kinetics Methanocaldococcus jannaschii
0.24
-
 tRNACys pH 7.5, 37°C, recombinant enzyme, m1G37 tRNACys Methanocaldococcus jannaschii
1
-
 tRNACys pH 7.5, 37°C, recombinant enzyme, native tRNACys Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanocaldococcus jannaschii