Any feedback?
Literature summary for 6.1.1.27 extracted from
- Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei (2008), J. Biol. Chem., 283, 22007-22017.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanosarcina mazei |
| expression of His-tagged enzyme in Escherichia coli | Methanosarcina mazei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-values for tRNACys isoacceptor | Methanosarcina mazei | |
| additional information | - |
additional information | steady-state aminoacylation kinetics | Methanosarcina mazei | |
| 0.0064 | - |
tRNACys | pH 7.5, 37°C, recombinant enzyme | Methanosarcina mazei |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Methanosarcina mazei | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + O-phospho-L-serine + tRNACys | Methanosarcina mazei | some methanogenic archaea synthesize Cys-tRNACys needed for protein synthesis using both a canonical cysteinyl-tRNA synthetase as well as a set of two enzymes that operate via a separate indirect pathway. In the indirect route, Sep-tRNACys is first synthesized by SepRS, and this misacylated intermediate is then converted to Cys-tRNACys by Sep-tRNA:Cys-tRNA synthase via a pyridoxal phosphate-dependent mechanism, structural basis for the tRNACys isoacceptor preferences of SepRS and CysRS, detailed overview | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina mazei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Methanosarcina mazei |
| recombinant His-tagged enzyme from Escherichia coli by anion exchange chromatography, gel filtration, and nickel affinity chromatography | Methanosarcina mazei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + O-phospho-L-serine + tRNACys | some methanogenic archaea synthesize Cys-tRNACys needed for protein synthesis using both a canonical cysteinyl-tRNA synthetase as well as a set of two enzymes that operate via a separate indirect pathway. In the indirect route, Sep-tRNACys is first synthesized by SepRS, and this misacylated intermediate is then converted to Cys-tRNACys by Sep-tRNA:Cys-tRNA synthase via a pyridoxal phosphate-dependent mechanism, structural basis for the tRNACys isoacceptor preferences of SepRS and CysRS, detailed overview | Methanosarcina mazei | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
| ATP + O-phospho-L-serine + tRNACys | efficient phosphoserylation by SepRS requires methylation of tRNACys at the N1 position of G37 in the anticodon loop. Comparative aminoacylation kinetics by CysRS (EC 6.1.1.16) and SepRS reveals that each enzyme prefers a distinct tRNACys isoacceptor or pair of isoacceptors | Methanosarcina mazei | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
| ATP + O-phospho-L-serine + tRNACys | recognition determinants distinguishing the tRNAs reside in the globular core of the molecule. The enzyme also requires the S-adenosylmethione-dependent formation of m1G37 in the anticodon loop for efficient aminoacylation | Methanosarcina mazei | AMP + diphosphate + O-phospho-L-seryl-tRNACys | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme is a class II tRNA synthetase and belongs to the PLP-dependent superfamily of enzymes | Methanosarcina mazei |
| phosphoseryl-tRNA synthetase | - |
Methanosarcina mazei |
| SepRS | - |
Methanosarcina mazei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanosarcina mazei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | turnover number for tRNACys isoacceptor | Methanosarcina mazei | |
| 0.12 | - |
tRNACys | pH 7.5, 37°C, recombinant enzyme | Methanosarcina mazei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Methanosarcina mazei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Methanosarcina mazei | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
