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Literature summary for 6.1.1.26 extracted from

  • Takimoto, J.K.; Dellas, N.; Noel, J.P.; Wang, L.
    Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids (2011), ACS Chem. Biol., 6, 733-743.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH10beta cells Methanosarcina mazei
expression in Escherichia coli Methanosarcina mazei

Crystallization (Commentary)

Crystallization (Comment) Organism
1.75 A X-ray crystal structure of the enzyme complexed with O-methyl-L-tyrosine and a non-hydrolyzable ATP analogue Methanosarcina mazei
mutant Y384F/A302T/N346V/C348W/V401L in complex with O-methyl-L-tyrosine, hanging drop vapor diffusion method, using Methanosarcina mazei

Protein Variants

Protein Variants Comment Organism
Y384F mutation increases the aminoacylation rate Methanosarcina mazei
Y384F the mutation increases the aminoacylation rate Methanosarcina mazei
Y384F/A302T/N346V/C348W/V401L the mutant specifically incorporates the cognate unnatural amino acid O-methyl-L-tyrosine into proteins Methanosarcina mazei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl Methanosarcina mazei
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
ATP + L-pyrrolysine + tRNAPyl Methanosarcina mazei DSM 3647
-
 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei Q8PWY1
-
-
Methanosarcina mazei DSM 3647 Q8PWY1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-pyrrolysine + tRNAPyl
-
 Methanosarcina mazei AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?
ATP + L-pyrrolysine + tRNAPyl
-
 Methanosarcina mazei DSM 3647 AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Methanosarcina mazei

Synonyms

Synonyms Comment Organism
PylRS
-
 Methanosarcina mazei
pyrrolysyl-tRNA synthetase
-
 Methanosarcina mazei

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanosarcina mazei